Entry Detail



General Information

Database ID:exR0000015
RNA Name:AAMP
RNA Type:mRNA
Chromosome:chr2
Starnd:-
Coordinate:
Start Site(bp):218264123End Site(bp):218270257
External Links:ENSG00000127837



Disease Information

Disease Name:Down Syndrome
Disease Category:Congenital, Hereditary, and Neonatal Diseases and Abnormalities
MeSH ID:D004314
Type:Diseases Category/Congenital, Hereditary, and Neonatal Diseases and Abnormalities
Alias:Syndrome, Down//Mongolism//47,XY,+21//Trisomy G//47,XX,+21//Down's Syndrome//Downs Syndrome//Syndrome, Down's//Trisomy 21//Trisomy 21, Mitotic Nondisjunction//Down Syndrome, Partial Trisomy 21//Partial Trisomy 21 Down Syndrome//Trisomy 21, Meiotic Nondisjunction



Expression Detail

GEO ID:GSE16176
Description:Expression profiles of amniotic fluid from human fetuses with Down syndrome and euploid controls
Experimental Design:Disease vs Control
Case Disease Type:Down Syndrome
Case Disease SubType:NA
Case Sample:Down Syndrome
Control Sample:Normal
Number of Case:7
Number of Control:7
Number of Samples:14





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ANKRD40
chr17
50693198
50707914
-
MOB1A
chr2
74152528
74178898
-
SF3A2
chr19
2236824
2248655
+
HSP90AA1
chr14
102080738
102139699
-
HSP90AB1
chr6
44246166
44253888
+
RPL10A
chr6
35468401
35470785
+
BOD1
chr5
173607145
173616659
-
FUS
chr16
31180110
31194871
+
RBMX
chrX
136848004
136880764
-
ELAC2
chr17
12991612
13018065
-
SF3B4
chr1
149923317
149927803
-
PLXNA1
chr3
126988594
127037392
+
IPO7
chr11
9384652
9448127
+
RABGAP1
chr9
122940833
123104866
+
NFKBIL1
chr6
31546870
31558829
+
MAP7D1
chr1
36155579
36180849
+
ANKH
chr5
14704800
14871778
-
KHDC4
chr1
155913045
155934413
-
SPSB4
chr3
141051347
141148611
+
WDR75
chr2
189441446
189475552
+
ACTB
chr7
5527148
5563784
-
NT5DC2
chr3
52524385
52535054
-
ITPR1
chr3
4493345
4847840
+
DHRS11
chr17
36591879
36600804
+
CRTAP
chr3
33114014
33147773
+
CCNG1
chr5
163437569
163446151
+
EEF1D
chr8
143579697
143599541
-
SOX2
chr3
181711925
181714436
+
XAB2
chr19
7619525
7629545
-
AGPAT5
chr8
6708642
6761503
+
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC073046.1
chr2
73985132
73986343
+
AD000090.1
chr19
35557956
35581954
+
AL022311.1
chr22
37876148
37895563
+
ARRDC1-AS1
chr9
137615332
137618906
-
BX284668.2
chr1
16870945
16883659
+
LINC01578
chr15
92819540
92899701
+
NEAT1
chr11
65422774
65445540
+
SNHG25
chr17
64145970
64146476
+
Display:



Experiment Detail

GEO ID:GSE16176
Sample Source:Amniotic Fluid
Source Fraction:Supernatant
Platform:GPL570
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:17063
Sample treatment protocol:NA
RNA Extract protocol:RNA was extracted from 10 mL AF with 30 mL TRIzol LS Reagent (Invitrogen, Carlsbad, CA) and 8 mL chloroform. After RNA extraction, RNA was purified and DNA was removed using the RNeasy® Maxi Kit, including the DNase step according to the manufacturer's protocol (QIAGEN, Valencia, CA). RNA was precipitated using 3M NaOAc and 100% ethanol, and 80% ethanol was added after 4 hours incubation at -20 °C. cDNA, synthesized from extracted RNA, was amplified and purified using the WT-Ovation™ Pico RNA Amplification System (NuGEN, San Carlos, CA) and the DNA Clean & Concentrator™-25 (Zymo Research, Orange, CA).
RNA library preparation protocol:biotin Samples were labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN, San Carlos, CA).



Reference

PMID:19474297
Title:Functional genomic analysis of amniotic fluid cell-free mRNA suggests that oxidative stress is significant in Down syndrome fetuses
Author:Slonim DK, Koide K, Johnson KL, Tantravahi U, Cowan JM, Jarrah Z, Bianchi DW
Journal:Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9425-9.
Description:To characterize the differences between second trimester Down syndrome (DS) and euploid fetuses, we used Affymetrix microarrays to compare gene expression in uncultured amniotic fluid supernatant samples.