exRNAdisease

General Information

Database ID:

exR0000233

RNA Name:

ADAM19

RNA Type:

mRNA

Chromosome:

chr5

Starnd:

Coordinate:

Start Site(bp):

157395534

End Site(bp):

157575775

External Links:

Disease Information

Disease Name:	Down Syndrome
Disease Category:	Congenital, Hereditary, and Neonatal Diseases and Abnormalities
MeSH ID:	D004314
Type:	Diseases Category/Congenital, Hereditary, and Neonatal Diseases and Abnormalities
Alias:	Syndrome, Down//Mongolism//47,XY,+21//Trisomy G//47,XX,+21//Down's Syndrome//Downs Syndrome//Syndrome, Down's//Trisomy 21//Trisomy 21, Mitotic Nondisjunction//Down Syndrome, Partial Trisomy 21//Partial Trisomy 21 Down Syndrome//Trisomy 21, Meiotic Nondisjunction

Expression Detail

GEO ID:	GSE16176
Description:	Expression profiles of amniotic fluid from human fetuses with Down syndrome and euploid controls
Experimental Design:	Disease vs Control
Case Disease Type:	Down Syndrome
Case Disease SubType:	NA
Case Sample:	Down Syndrome
Control Sample:	Normal
Number of Case:	7
Number of Control:	7
Number of Samples:	14

Regulatory Relationship

mRNA targets:

Gene Symbol	Chromosome	Start Site(bp)	End Site(bp)	Strand
AGO4	chr1	35808016	35857890	+
AICDA	chr12	8602170	8612867	-
BZW1	chr2	200810594	200827338	+
CALM3	chr19	46601074	46610782	+
DDX17	chr22	38483438	38507660	-
EIF3J	chr15	44537125	44563029	+
FKBP1A	chr20	1368978	1393172	-
FOCAD	chr9	20658309	20995955	+
GTF3C2	chr2	27325849	27357034	-
IGFBP2	chr2	216632828	216664436	+
LRP5L	chr22	25351418	25405377	-
MGST3	chr1	165631213	165661796	+
MYH9	chr22	36281277	36388067	-
NDST1	chr5	150485818	150558211	+
PRDX5	chr11	64318121	64321811	+
PSMD8	chr19	38374536	38383824	+
PTPN6	chr12	6946468	6961316	+
RAB1B	chr11	66268590	66277492	+
RNF5	chr6	32178405	32180793	+
RPL13	chr16	89560657	89566828	+
SLC38A1	chr12	46183063	46270017	-
SMARCA4	chr19	10960825	11079426	+
STMN1	chr1	25884181	25906991	-
TATDN2	chr3	10248023	10281218	+
TMEM60	chr7	77793728	77798434	-
VPS18	chr15	40894450	40903975	+
ZNF354B	chr5	178859953	178888122	+
ZNHIT2	chr11	65116403	65117701	-

miRNA targets:

miRNA Symbol	Chromosome	Start Site(bp)	End Site(bp)	Strand
hsa-miR-423-5p	chr17	30117095	30117117	+

circRNA targets:

lncRNA targets:

Display:

Experiment Detail

GEO ID:	GSE16176
Sample Source:	Amniotic Fluid
Source Fraction:	Supernatant
Platform:	GPL570
Method:	Microarray
Num of detected RNA Type:	1
Num of detected RNAs of this Type:	17063
Sample treatment protocol:	NA
RNA Extract protocol:	RNA was extracted from 10 mL AF with 30 mL TRIzol LS Reagent (Invitrogen, Carlsbad, CA) and 8 mL chloroform. After RNA extraction, RNA was purified and DNA was removed using the RNeasy® Maxi Kit, including the DNase step according to the manufacturer's protocol (QIAGEN, Valencia, CA). RNA was precipitated using 3M NaOAc and 100% ethanol, and 80% ethanol was added after 4 hours incubation at -20 °C. cDNA, synthesized from extracted RNA, was amplified and purified using the WT-Ovation™ Pico RNA Amplification System (NuGEN, San Carlos, CA) and the DNA Clean & Concentrator™-25 (Zymo Research, Orange, CA).
RNA library preparation protocol:	biotin Samples were labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN, San Carlos, CA).

Reference

PMID:	19474297
Title:	Functional genomic analysis of amniotic fluid cell-free mRNA suggests that oxidative stress is significant in Down syndrome fetuses
Author:	Slonim DK, Koide K, Johnson KL, Tantravahi U, Cowan JM, Jarrah Z, Bianchi DW
Journal:	Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9425-9.
Description:	To characterize the differences between second trimester Down syndrome (DS) and euploid fetuses, we used Affymetrix microarrays to compare gene expression in uncultured amniotic fluid supernatant samples.