exRNAdisease

General Information

Database ID:

exR0000283

RNA Name:

ADCY1

RNA Type:

mRNA

Chromosome:

chr7

Starnd:

Coordinate:

Start Site(bp):

45574140

End Site(bp):

45723116

External Links:

Disease Information

Disease Name:	Down Syndrome
Disease Category:	Congenital, Hereditary, and Neonatal Diseases and Abnormalities
MeSH ID:	D004314
Type:	Diseases Category/Congenital, Hereditary, and Neonatal Diseases and Abnormalities
Alias:	Syndrome, Down//Mongolism//47,XY,+21//Trisomy G//47,XX,+21//Down's Syndrome//Downs Syndrome//Syndrome, Down's//Trisomy 21//Trisomy 21, Mitotic Nondisjunction//Down Syndrome, Partial Trisomy 21//Partial Trisomy 21 Down Syndrome//Trisomy 21, Meiotic Nondisjunction

Expression Detail

GEO ID:	GSE16176
Description:	Expression profiles of amniotic fluid from human fetuses with Down syndrome and euploid controls
Experimental Design:	Disease vs Control
Case Disease Type:	Down Syndrome
Case Disease SubType:	NA
Case Sample:	Down Syndrome
Control Sample:	Normal
Number of Case:	7
Number of Control:	7
Number of Samples:	14

Regulatory Relationship

mRNA targets:

Gene Symbol	Chromosome	Start Site(bp)	End Site(bp)	Strand
HLA-A	chr6	29941260	29945884	+
RBMXL1	chr1	88979456	88992960	-
CTTN	chr11	70398404	70436584	+
SCYL1	chr11	65525077	65538704	+
FP565260.1	chr21	5130871	5154658	-
ICE1	chr5	5420664	5490220	+
GNAS	chr20	58839718	58911192	+
NFATC2IP	chr16	28950807	28967097	+
SV2A	chr1	149903318	149917844	-
GLRX3	chr10	130136391	130184521	+
LAMA5	chr20	62307955	62367312	-
MFSD1	chr3	158732198	158829719	+
TMEM185A	chrX	149596556	149631912	-
UBXN4	chr2	135741734	135785056	+
C6orf62	chr6	24704861	24719998	-
SZRD1	chr1	16352575	16398145	+
PWP2	chr21	44107373	44131181	+
MBP	chr18	76978827	77133683	-
ATP5MC2	chr12	53632726	53677408	-
KLF2	chr19	16324817	16327874	+
GAPDH	chr12	6534512	6538374	+
KAT2A	chr17	42113111	42121367	-
HUWE1	chrX	53532096	53686728	-
RAB5A	chr3	19947097	19985175	+
PHB2	chr12	6965327	6970753	-
MARCKS	chr6	113857345	113863475	+
SUMO3	chr21	44805617	44818779	-
NCOA6	chr20	34689097	34825651	-
FBRSL1	chr12	132489551	132585188	+
LAMB1	chr7	107923799	108003187	-

miRNA targets:

circRNA targets:

lncRNA targets:

lncRNA Symbol	Chromosome	Start Site(bp)	End Site(bp)	Strand
LINC00665	chr19	36313067	36331770	-
XIST	chrX	73820649	73852723	-

Display:

Experiment Detail

GEO ID:	GSE16176
Sample Source:	Amniotic Fluid
Source Fraction:	Supernatant
Platform:	GPL570
Method:	Microarray
Num of detected RNA Type:	1
Num of detected RNAs of this Type:	17063
Sample treatment protocol:	NA
RNA Extract protocol:	RNA was extracted from 10 mL AF with 30 mL TRIzol LS Reagent (Invitrogen, Carlsbad, CA) and 8 mL chloroform. After RNA extraction, RNA was purified and DNA was removed using the RNeasy® Maxi Kit, including the DNase step according to the manufacturer's protocol (QIAGEN, Valencia, CA). RNA was precipitated using 3M NaOAc and 100% ethanol, and 80% ethanol was added after 4 hours incubation at -20 °C. cDNA, synthesized from extracted RNA, was amplified and purified using the WT-Ovation™ Pico RNA Amplification System (NuGEN, San Carlos, CA) and the DNA Clean & Concentrator™-25 (Zymo Research, Orange, CA).
RNA library preparation protocol:	biotin Samples were labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN, San Carlos, CA).

Reference

PMID:	19474297
Title:	Functional genomic analysis of amniotic fluid cell-free mRNA suggests that oxidative stress is significant in Down syndrome fetuses
Author:	Slonim DK, Koide K, Johnson KL, Tantravahi U, Cowan JM, Jarrah Z, Bianchi DW
Journal:	Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9425-9.
Description:	To characterize the differences between second trimester Down syndrome (DS) and euploid fetuses, we used Affymetrix microarrays to compare gene expression in uncultured amniotic fluid supernatant samples.