exRNAdisease

General Information

Database ID:

exR0000321

RNA Name:

ADORA2A

RNA Type:

mRNA

Chromosome:

chr22

Starnd:

Coordinate:

Start Site(bp):

24417879

End Site(bp):

24442357

External Links:

Disease Information

Disease Name:	Down Syndrome
Disease Category:	Congenital, Hereditary, and Neonatal Diseases and Abnormalities
MeSH ID:	D004314
Type:	Diseases Category/Congenital, Hereditary, and Neonatal Diseases and Abnormalities
Alias:	Syndrome, Down//Mongolism//47,XY,+21//Trisomy G//47,XX,+21//Down's Syndrome//Downs Syndrome//Syndrome, Down's//Trisomy 21//Trisomy 21, Mitotic Nondisjunction//Down Syndrome, Partial Trisomy 21//Partial Trisomy 21 Down Syndrome//Trisomy 21, Meiotic Nondisjunction

Expression Detail

GEO ID:	GSE16176
Description:	Expression profiles of amniotic fluid from human fetuses with Down syndrome and euploid controls
Experimental Design:	Disease vs Control
Case Disease Type:	Down Syndrome
Case Disease SubType:	NA
Case Sample:	Down Syndrome
Control Sample:	Normal
Number of Case:	7
Number of Control:	7
Number of Samples:	14

Regulatory Relationship

mRNA targets:

Gene Symbol	Chromosome	Start Site(bp)	End Site(bp)	Strand
TUBA1A	chr12	49184795	49189080	-
ILK	chr11	6603708	6610874	+
RPL23A	chr17	28719985	28724359	+
TUB	chr11	8019244	8106112	+
GABARAP	chr17	7240008	7242449	-
RETREG2	chr2	219176225	219185475	+
RPS3	chr11	75399515	75422280	+
LMNA	chr1	156082573	156140089	+
SPECC1L	chr22	24270817	24417739	+
IFI30	chr19	18173162	18178117	+
NFKB2	chr10	102394110	102402524	+
RPL9	chr4	39452521	39458949	-
MX1	chr21	41420304	41459214	+
EIF4B	chr12	53006158	53042209	+
LRFN4	chr11	66856647	66860475	+
CTSH	chr15	78921058	78949574	-
CHCHD3	chr7	132784870	133082090	-
ITGB1	chr10	32900318	33005792	-
MTPN	chr7	135926760	135977359	-
IFFO1	chr12	6538375	6556083	-
CLK3	chr15	74598500	74645414	+
RPL3	chr22	39312882	39320389	-
P4HB	chr17	81843161	81860624	-
RPS16	chr19	39433137	39435949	-
VAMP5	chr2	85584431	85593406	+
SPECC1L-ADORA2A	chr22	24270898	24442356	+
TMSB4X	chrX	12975110	12977227	+
MCM2	chr3	127598410	127622436	+
ACTG1	chr17	81509971	81523847	-
EIF3D	chr22	36510855	36529184	-

miRNA targets:

circRNA targets:

lncRNA targets:

lncRNA Symbol	Chromosome	Start Site(bp)	End Site(bp)	Strand
AL121832.3	chr20	62402236	62405935	-
AL137127.1	chr1	19072110	19075511	-

Display:

Experiment Detail

GEO ID:	GSE16176
Sample Source:	Amniotic Fluid
Source Fraction:	Supernatant
Platform:	GPL570
Method:	Microarray
Num of detected RNA Type:	1
Num of detected RNAs of this Type:	17063
Sample treatment protocol:	NA
RNA Extract protocol:	RNA was extracted from 10 mL AF with 30 mL TRIzol LS Reagent (Invitrogen, Carlsbad, CA) and 8 mL chloroform. After RNA extraction, RNA was purified and DNA was removed using the RNeasy® Maxi Kit, including the DNase step according to the manufacturer's protocol (QIAGEN, Valencia, CA). RNA was precipitated using 3M NaOAc and 100% ethanol, and 80% ethanol was added after 4 hours incubation at -20 °C. cDNA, synthesized from extracted RNA, was amplified and purified using the WT-Ovation™ Pico RNA Amplification System (NuGEN, San Carlos, CA) and the DNA Clean & Concentrator™-25 (Zymo Research, Orange, CA).
RNA library preparation protocol:	biotin Samples were labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN, San Carlos, CA).

Reference

PMID:	19474297
Title:	Functional genomic analysis of amniotic fluid cell-free mRNA suggests that oxidative stress is significant in Down syndrome fetuses
Author:	Slonim DK, Koide K, Johnson KL, Tantravahi U, Cowan JM, Jarrah Z, Bianchi DW
Journal:	Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9425-9.
Description:	To characterize the differences between second trimester Down syndrome (DS) and euploid fetuses, we used Affymetrix microarrays to compare gene expression in uncultured amniotic fluid supernatant samples.