exRNAdisease

General Information

Database ID:

exR0004522

RNA Name:

ENO1

RNA Type:

mRNA

Chromosome:

chr1

Starnd:

Coordinate:

Start Site(bp):

8861000

End Site(bp):

8879190

External Links:

Disease Information

Disease Name:	Down Syndrome
Disease Category:	Congenital, Hereditary, and Neonatal Diseases and Abnormalities
MeSH ID:	D004314
Type:	Diseases Category/Congenital, Hereditary, and Neonatal Diseases and Abnormalities
Alias:	Syndrome, Down//Mongolism//47,XY,+21//Trisomy G//47,XX,+21//Down's Syndrome//Downs Syndrome//Syndrome, Down's//Trisomy 21//Trisomy 21, Mitotic Nondisjunction//Down Syndrome, Partial Trisomy 21//Partial Trisomy 21 Down Syndrome//Trisomy 21, Meiotic Nondisjunction

Expression Detail

GEO ID:	GSE16176
Description:	Expression profiles of amniotic fluid from human fetuses with Down syndrome and euploid controls
Experimental Design:	Disease vs Control
Case Disease Type:	Down Syndrome
Case Disease SubType:	NA
Case Sample:	Down Syndrome
Control Sample:	Normal
Number of Case:	7
Number of Control:	7
Number of Samples:	14

Regulatory Relationship

mRNA targets:

Gene Symbol	Chromosome	Start Site(bp)	End Site(bp)	Strand
AL049629.2	chr11	33698261	33722467	-
ACAD10	chr12	111686056	111757107	+
ACSS2	chr20	34872146	34927962	+
AC004832.3	chr22	30409255	30428990	+
AC048338.1	chr12	122207779	122266423	-
AGFG1	chr2	227472152	227561214	+
AHSA1	chr14	77457870	77469472	+
AC010422.5	chr19	12664828	12669397	-
AC010463.1	chr19	17267418	17282966	+
AKR1A1	chr1	45550543	45570049	+
AC011448.1	chr19	19516227	19536076	+
AHNAK	chr11	62433542	62556235	-
ACTG1	chr17	81509971	81523847	-
AL136295.5	chr14	24147548	24166452	+
AC091167.2	chr15	90249556	90272208	+
ALDH16A1	chr19	49453225	49471050	+
ACTA1	chr1	229431245	229434098	-
AC010422.3	chr19	12643831	12648397	-
AC073283.3	chr2	47065941	47176511	-
AL136454.1	chr1	192716132	192716653	+
ABRACL	chr6	139028745	139043302	+
ALDH18A1	chr10	95605941	95656711	-
ABCC1	chr16	15949577	16143074	+
AC138811.2	chr16	18788063	18801519	-
AC018523.2	chr11	14493783	14520344	-
AL033529.1	chr1	32465057	32600792	+
AC107871.1	chr15	68184032	68229718	-
ACIN1	chr14	23058564	23095614	-
AHCY	chr20	34280268	34311802	-
AAGAB	chr15	67200667	67255195	-

miRNA targets:

miRNA Symbol	Chromosome	Start Site(bp)	End Site(bp)	Strand
hsa-miR-103a-3p	chr5	168560904	168560926	-
hsa-miR-423-3p	chr17	30117131	30117153	+
hsa-miR-4426	chr1	192716333	192716349	+
hsa-miR-6728-3p	chr1	8866502	8866522	-

circRNA targets:

lncRNA targets:

lncRNA Symbol	Chromosome	Start Site(bp)	End Site(bp)	Strand
AC004687.1	chr17	58330884	58332508	-
AC005261.3	chr19	57350848	57352012	-
AC005899.4	chr17	32328441	32329395	+
AC007952.4	chr17	19112000	19112636	-
AC008124.1	chr12	45718046	45727775	-
AC010655.2	chr7	128455937	128469197	+
AC010655.4	chr7	128455840	128493859	+
AC016876.2	chr17	7581964	7584086	-
AC021078.1	chr5	149494314	149504670	-
AC023509.1	chr12	53441741	53467528	+
AC026362.1	chr12	122975320	122982907	+
AC026401.3	chr16	15701237	15702118	+
AC079781.5	chr7	97851688	97972985	-
AC091057.1	chr15	30658717	30772993	+
AC091564.7	chr11	6610883	6616594	-
AC097478.1	chr4	89743792	89744305	+
AC109460.3	chr16	28974804	28990775	+
AC124068.2	chr15	89335053	89336161	+
AC132217.1	chr11	2129121	2129964	-
AC245014.3	chr1	145281116	145281462	+
AD000090.1	chr19	35557956	35581954	+
AL022311.1	chr22	37876148	37895563	+
AL121832.2	chr20	62386303	62386970	-
AL132780.1	chr14	22929607	22956374	+

Display:

Experiment Detail

GEO ID:	GSE16176
Sample Source:	Amniotic Fluid
Source Fraction:	Supernatant
Platform:	GPL570
Method:	Microarray
Num of detected RNA Type:	1
Num of detected RNAs of this Type:	17063
Sample treatment protocol:	NA
RNA Extract protocol:	RNA was extracted from 10 mL AF with 30 mL TRIzol LS Reagent (Invitrogen, Carlsbad, CA) and 8 mL chloroform. After RNA extraction, RNA was purified and DNA was removed using the RNeasy® Maxi Kit, including the DNase step according to the manufacturer's protocol (QIAGEN, Valencia, CA). RNA was precipitated using 3M NaOAc and 100% ethanol, and 80% ethanol was added after 4 hours incubation at -20 °C. cDNA, synthesized from extracted RNA, was amplified and purified using the WT-Ovation™ Pico RNA Amplification System (NuGEN, San Carlos, CA) and the DNA Clean & Concentrator™-25 (Zymo Research, Orange, CA).
RNA library preparation protocol:	biotin Samples were labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN, San Carlos, CA).

Reference

PMID:	19474297
Title:	Functional genomic analysis of amniotic fluid cell-free mRNA suggests that oxidative stress is significant in Down syndrome fetuses
Author:	Slonim DK, Koide K, Johnson KL, Tantravahi U, Cowan JM, Jarrah Z, Bianchi DW
Journal:	Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9425-9.
Description:	To characterize the differences between second trimester Down syndrome (DS) and euploid fetuses, we used Affymetrix microarrays to compare gene expression in uncultured amniotic fluid supernatant samples.