exRNAdisease

Entry Detail

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General Information

Database ID: exR0004788 RNA Name: FAM102A RNA Type: mRNA Chromosome: chr9 Starnd: - Coordinate: Start Site(bp): 127940582 End Site(bp): 127980989 External Links: ENSG00000167106

Disease Information

Disease Name: Down Syndrome Disease Category: Congenital, Hereditary, and Neonatal Diseases and Abnormalities MeSH ID: D004314 Type: Diseases Category/Congenital, Hereditary, and Neonatal Diseases and Abnormalities Alias: Syndrome, Down//Mongolism//47,XY,+21//Trisomy G//47,XX,+21//Down's Syndrome//Downs Syndrome//Syndrome, Down's//Trisomy 21//Trisomy 21, Mitotic Nondisjunction//Down Syndrome, Partial Trisomy 21//Partial Trisomy 21 Down Syndrome//Trisomy 21, Meiotic Nondisjunction

Expression Detail

GEO ID: GSE16176 Description: Expression profiles of amniotic fluid from human fetuses with Down syndrome and euploid controls Experimental Design: Disease vs Control Case Disease Type: Down Syndrome Case Disease SubType: NA Case Sample: Down Syndrome Control Sample: Normal Number of Case: 7 Number of Control: 7 Number of Samples: 14

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ACADVL chr17 7217125 7225266 + AHCY chr20 34280268 34311802 - AKAP1 chr17 57085092 57121346 + ALDOA chr16 30064164 30070457 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-17-5p chr13 91350618 91350640 + hsa-miR-28-5p chr3 188688794 188688815 + hsa-miR-122-5p chr18 58451088 58451109 + hsa-miR-124-3p chr8 64379210 64379231 + hsa-miR-130a-3p chr11 57641252 57641273 + hsa-miR-302a-3p chr4 112648186 112648208 - hsa-miR-130b-3p chr22 21653354 21653375 + hsa-miR-302c-3p chr4 112648366 112648388 - hsa-miR-20b-5p chrX 134169850 134169872 - hsa-miR-485-5p chr14 101055427 101055448 + hsa-miR-520a-5p chr19 53690895 53690915 + hsa-miR-519b-3p chr19 53695263 53695284 + hsa-miR-520d-3p chr19 53720149 53720170 + hsa-miR-625-5p chr14 65471116 65471136 + hsa-miR-650 chr22 22822791 22822811 + hsa-miR-654-5p chr14 101040234 101040255 + hsa-miR-193a-5p chr17 31560016 31560037 + hsa-miR-423-5p chr17 30117095 30117117 + hsa-miR-574-5p chr4 38868056 38868078 + hsa-miR-541-3p chr14 101064548 101064569 + hsa-miR-708-5p chr11 79402077 79402099 - circRNA targets: NA lncRNA targets: NA Display:

Experiment Detail

GEO ID: GSE16176 Sample Source: Amniotic Fluid Source Fraction: Supernatant Platform: GPL570 Method: Microarray Num of detected RNA Type: 1 Num of detected RNAs of this Type: 17063 Sample treatment protocol: NA RNA Extract protocol: RNA was extracted from 10 mL AF with 30 mL TRIzol LS Reagent (Invitrogen, Carlsbad, CA) and 8 mL chloroform. After RNA extraction, RNA was purified and DNA was removed using the RNeasy® Maxi Kit, including the DNase step according to the manufacturer's protocol (QIAGEN, Valencia, CA). RNA was precipitated using 3M NaOAc and 100% ethanol, and 80% ethanol was added after 4 hours incubation at -20 °C. cDNA, synthesized from extracted RNA, was amplified and purified using the WT-Ovation™ Pico RNA Amplification System (NuGEN, San Carlos, CA) and the DNA Clean & Concentrator™-25 (Zymo Research, Orange, CA). RNA library preparation protocol: biotin Samples were labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN, San Carlos, CA).

Reference

PMID: 19474297 Title: Functional genomic analysis of amniotic fluid cell-free mRNA suggests that oxidative stress is significant in Down syndrome fetuses Author: Slonim DK, Koide K, Johnson KL, Tantravahi U, Cowan JM, Jarrah Z, Bianchi DW Journal: Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9425-9. Description: To characterize the differences between second trimester Down syndrome (DS) and euploid fetuses, we used Affymetrix microarrays to compare gene expression in uncultured amniotic fluid supernatant samples.