exRNAdisease

Entry Detail

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General Information

Database ID: exR0005009 RNA Name: FBL RNA Type: mRNA Chromosome: chr19 Starnd: - Coordinate: Start Site(bp): 39834458 End Site(bp): 39846379 External Links: ENSG00000105202

Disease Information

Disease Name: Down Syndrome Disease Category: Congenital, Hereditary, and Neonatal Diseases and Abnormalities MeSH ID: D004314 Type: Diseases Category/Congenital, Hereditary, and Neonatal Diseases and Abnormalities Alias: Syndrome, Down//Mongolism//47,XY,+21//Trisomy G//47,XX,+21//Down's Syndrome//Downs Syndrome//Syndrome, Down's//Trisomy 21//Trisomy 21, Mitotic Nondisjunction//Down Syndrome, Partial Trisomy 21//Partial Trisomy 21 Down Syndrome//Trisomy 21, Meiotic Nondisjunction

Expression Detail

GEO ID: GSE16176 Description: Expression profiles of amniotic fluid from human fetuses with Down syndrome and euploid controls Experimental Design: Disease vs Control Case Disease Type: Down Syndrome Case Disease SubType: NA Case Sample: Down Syndrome Control Sample: Normal Number of Case: 7 Number of Control: 7 Number of Samples: 14

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABCC8 chr11 17392498 17476879 - ABHD12 chr20 25294742 25390835 - AC006064.6 chr12 6556886 6607367 - AC008878.2 chr19 7533595 7540059 + AC010323.1 chr19 8308283 8321379 - AC010422.3 chr19 12643831 12648397 - AC092143.1 chr16 89919165 89936092 + AC116366.3 chr5 132410832 132646079 + AC138811.2 chr16 18788063 18801519 - AC245033.1 chr15 82536788 82573194 - ACADVL chr17 7217125 7225266 + ACLY chr17 41866917 41930542 - ACSL1 chr4 184755595 184826818 - ACTB chr7 5527148 5563784 - ACTG1 chr17 81509971 81523847 - ACVR1B chr12 51951699 51997078 + AEN chr15 88621337 88632281 + AGAP2 chr12 57723761 57742157 - AKIRIN2 chr6 87674860 87702233 - AKR1A1 chr1 45550543 45570049 + AL160269.1 chr9 96235331 96352058 - ALDH18A1 chr10 95605941 95656711 - ALDOA chr16 30064164 30070457 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC067930.1 chr8 143579636 143580670 + AC245014.3 chr1 145281116 145281462 + AD000090.1 chr19 35557956 35581954 + Display:

Experiment Detail

GEO ID: GSE16176 Sample Source: Amniotic Fluid Source Fraction: Supernatant Platform: GPL570 Method: Microarray Num of detected RNA Type: 1 Num of detected RNAs of this Type: 17063 Sample treatment protocol: NA RNA Extract protocol: RNA was extracted from 10 mL AF with 30 mL TRIzol LS Reagent (Invitrogen, Carlsbad, CA) and 8 mL chloroform. After RNA extraction, RNA was purified and DNA was removed using the RNeasy® Maxi Kit, including the DNase step according to the manufacturer's protocol (QIAGEN, Valencia, CA). RNA was precipitated using 3M NaOAc and 100% ethanol, and 80% ethanol was added after 4 hours incubation at -20 °C. cDNA, synthesized from extracted RNA, was amplified and purified using the WT-Ovation™ Pico RNA Amplification System (NuGEN, San Carlos, CA) and the DNA Clean & Concentrator™-25 (Zymo Research, Orange, CA). RNA library preparation protocol: biotin Samples were labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN, San Carlos, CA).

Reference

PMID: 19474297 Title: Functional genomic analysis of amniotic fluid cell-free mRNA suggests that oxidative stress is significant in Down syndrome fetuses Author: Slonim DK, Koide K, Johnson KL, Tantravahi U, Cowan JM, Jarrah Z, Bianchi DW Journal: Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9425-9. Description: To characterize the differences between second trimester Down syndrome (DS) and euploid fetuses, we used Affymetrix microarrays to compare gene expression in uncultured amniotic fluid supernatant samples.