| GEO ID: | GSE16176 | | Sample Source: | Amniotic Fluid | | Source Fraction: | Supernatant | | Platform: | GPL570 | | Method: | Microarray | | Num of detected RNA Type: | 1 | | Num of detected RNAs of this Type: | 17063 | | Sample treatment protocol: | NA | | RNA Extract protocol: | RNA was extracted from 10 mL AF with 30 mL TRIzol LS Reagent (Invitrogen, Carlsbad, CA) and 8 mL chloroform. After RNA extraction, RNA was purified and DNA was removed using the RNeasy® Maxi Kit, including the DNase step according to the manufacturer's protocol (QIAGEN, Valencia, CA). RNA was precipitated using 3M NaOAc and 100% ethanol, and 80% ethanol was added after 4 hours incubation at -20 °C. cDNA, synthesized from extracted RNA, was amplified and purified using the WT-Ovation™ Pico RNA Amplification System (NuGEN, San Carlos, CA) and the DNA Clean & Concentrator™-25 (Zymo Research, Orange, CA). | | RNA library preparation protocol: | biotin Samples were labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN, San Carlos, CA). |
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