exRNAdisease

Entry Detail

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General Information

Database ID: exR0009875 RNA Name: OAZ1 RNA Type: mRNA Chromosome: chr19 Starnd: + Coordinate: Start Site(bp): 2269509 End Site(bp): 2273490 External Links: ENSG00000104904

Disease Information

Disease Name: Down Syndrome Disease Category: Congenital, Hereditary, and Neonatal Diseases and Abnormalities MeSH ID: D004314 Type: Diseases Category/Congenital, Hereditary, and Neonatal Diseases and Abnormalities Alias: Syndrome, Down//Mongolism//47,XY,+21//Trisomy G//47,XX,+21//Down's Syndrome//Downs Syndrome//Syndrome, Down's//Trisomy 21//Trisomy 21, Mitotic Nondisjunction//Down Syndrome, Partial Trisomy 21//Partial Trisomy 21 Down Syndrome//Trisomy 21, Meiotic Nondisjunction

Expression Detail

GEO ID: GSE16176 Description: Expression profiles of amniotic fluid from human fetuses with Down syndrome and euploid controls Experimental Design: Disease vs Control Case Disease Type: Down Syndrome Case Disease SubType: NA Case Sample: Down Syndrome Control Sample: Normal Number of Case: 7 Number of Control: 7 Number of Samples: 14

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AAGAB chr15 67200667 67255195 - ABCB8 chr7 151028422 151047782 + ABL2 chr1 179099330 179229684 - AC005258.1 chr19 2269525 2341172 + AC006030.1 chr2 74211604 74363377 - AC008982.1 chr19 38817471 38840178 - AC010422.3 chr19 12643831 12648397 - AC011455.2 chr19 38915404 38949855 - AC073508.2 chr17 40627356 40665141 - AC104109.3 chr5 134167170 134226071 - AC124312.1 chr15 24955034 25000276 + ACAT2 chr6 159762045 159779112 + ACLY chr17 41866917 41930542 - ACTB chr7 5527148 5563784 - ACTG1 chr17 81509971 81523847 - ACTL6A chr3 179562886 179588407 + ACTN1 chr14 68874143 68979440 - ACTR8 chr3 53867066 53882152 - ADAR chr1 154582057 154628013 - AEN chr15 88621337 88632281 + AHCTF1 chr1 246839098 246931967 - AHSA1 chr14 77457870 77469472 + AIFM1 chrX 130129362 130165887 - AKAP1 chr17 57085092 57121346 + AL133352.1 chr10 100505628 100529881 - AL139260.3 chr1 38864501 38881617 - ALCAM chr3 105366909 105576900 + ALDOA chr16 30064164 30070457 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC007952.4 chr17 19112000 19112636 - AC009908.1 chr8 124996985 124998198 - AC067930.1 chr8 143579636 143580670 + AC079145.1 chr2 19990209 20004795 + AC087392.5 chr17 795306 795794 + AD000090.1 chr19 35557956 35581954 + Display:

Experiment Detail

GEO ID: GSE16176 Sample Source: Amniotic Fluid Source Fraction: Supernatant Platform: GPL570 Method: Microarray Num of detected RNA Type: 1 Num of detected RNAs of this Type: 17063 Sample treatment protocol: NA RNA Extract protocol: RNA was extracted from 10 mL AF with 30 mL TRIzol LS Reagent (Invitrogen, Carlsbad, CA) and 8 mL chloroform. After RNA extraction, RNA was purified and DNA was removed using the RNeasy® Maxi Kit, including the DNase step according to the manufacturer's protocol (QIAGEN, Valencia, CA). RNA was precipitated using 3M NaOAc and 100% ethanol, and 80% ethanol was added after 4 hours incubation at -20 °C. cDNA, synthesized from extracted RNA, was amplified and purified using the WT-Ovation™ Pico RNA Amplification System (NuGEN, San Carlos, CA) and the DNA Clean & Concentrator™-25 (Zymo Research, Orange, CA). RNA library preparation protocol: biotin Samples were labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN, San Carlos, CA).

Reference

PMID: 19474297 Title: Functional genomic analysis of amniotic fluid cell-free mRNA suggests that oxidative stress is significant in Down syndrome fetuses Author: Slonim DK, Koide K, Johnson KL, Tantravahi U, Cowan JM, Jarrah Z, Bianchi DW Journal: Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9425-9. Description: To characterize the differences between second trimester Down syndrome (DS) and euploid fetuses, we used Affymetrix microarrays to compare gene expression in uncultured amniotic fluid supernatant samples.