exRNAdisease

General Information

Database ID:

exR0012329

RNA Name:

RPL18A

RNA Type:

mRNA

Chromosome:

chr19

Starnd:

Coordinate:

Start Site(bp):

17859910

End Site(bp):

17864153

External Links:

Disease Information

Disease Name:	Down Syndrome
Disease Category:	Congenital, Hereditary, and Neonatal Diseases and Abnormalities
MeSH ID:	D004314
Type:	Diseases Category/Congenital, Hereditary, and Neonatal Diseases and Abnormalities
Alias:	Syndrome, Down//Mongolism//47,XY,+21//Trisomy G//47,XX,+21//Down's Syndrome//Downs Syndrome//Syndrome, Down's//Trisomy 21//Trisomy 21, Mitotic Nondisjunction//Down Syndrome, Partial Trisomy 21//Partial Trisomy 21 Down Syndrome//Trisomy 21, Meiotic Nondisjunction

Expression Detail

GEO ID:	GSE16176
Description:	Expression profiles of amniotic fluid from human fetuses with Down syndrome and euploid controls
Experimental Design:	Disease vs Control
Case Disease Type:	Down Syndrome
Case Disease SubType:	NA
Case Sample:	Down Syndrome
Control Sample:	Normal
Number of Case:	7
Number of Control:	7
Number of Samples:	14

Regulatory Relationship

mRNA targets:

Gene Symbol	Chromosome	Start Site(bp)	End Site(bp)	Strand
AL928654.3	chr14	105487199	105492267	+
ALDOA	chr16	30064164	30070457	+
AC073508.2	chr17	40627356	40665141	-
ADGRV1	chr5	90529344	91164437	+
ACAA2	chr18	49782164	49813953	-
AKT1	chr14	104769349	104795751	-
AGPAT5	chr8	6708642	6761503	+
ADD1	chr4	2843857	2930076	+
ABCC10	chr6	43427366	43450427	+
ACTB	chr7	5527148	5563784	-
AC010422.3	chr19	12643831	12648397	-
ABHD5	chr3	43690108	43734371	+
ADAM10	chr15	58588809	58749791	-
ACTN4	chr19	38647649	38731589	+
AASS	chr7	122073549	122144255	-
AGO2	chr8	140520156	140635633	-
AC008982.1	chr19	38817471	38840178	-
ACSF3	chr16	89088375	89164121	+
AHCY	chr20	34280268	34311802	-
ACLY	chr17	41866917	41930542	-
AL359922.1	chr9	21802636	22029594	+
AC104109.3	chr5	134167170	134226071	-
AL358113.1	chr9	69035747	69255187	+
ADAMTS10	chr19	8580240	8610735	-
AC093525.2	chr16	2496032	2520218	+
AC004922.1	chr7	99325879	99394653	+
ABRAXAS2	chr10	124801819	124836667	+
ACADVL	chr17	7217125	7225266	+
AL022318.4	chr22	39014363	39033276	+
AC093525.1	chr16	2513965	2527955	+

miRNA targets:

circRNA targets:

lncRNA targets:

lncRNA Symbol	Chromosome	Start Site(bp)	End Site(bp)	Strand
AC245297.2	chr1	149264252	149264816	+
AD000090.1	chr19	35557956	35581954	+
AL021154.1	chr1	23549139	23550915	-
AL117378.1	chr6	131901963	131920565	+
AL137782.1	chr13	75549773	75807120	+

Display:

Experiment Detail

GEO ID:	GSE16176
Sample Source:	Amniotic Fluid
Source Fraction:	Supernatant
Platform:	GPL570
Method:	Microarray
Num of detected RNA Type:	1
Num of detected RNAs of this Type:	17063
Sample treatment protocol:	NA
RNA Extract protocol:	RNA was extracted from 10 mL AF with 30 mL TRIzol LS Reagent (Invitrogen, Carlsbad, CA) and 8 mL chloroform. After RNA extraction, RNA was purified and DNA was removed using the RNeasy® Maxi Kit, including the DNase step according to the manufacturer's protocol (QIAGEN, Valencia, CA). RNA was precipitated using 3M NaOAc and 100% ethanol, and 80% ethanol was added after 4 hours incubation at -20 °C. cDNA, synthesized from extracted RNA, was amplified and purified using the WT-Ovation™ Pico RNA Amplification System (NuGEN, San Carlos, CA) and the DNA Clean & Concentrator™-25 (Zymo Research, Orange, CA).
RNA library preparation protocol:	biotin Samples were labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN, San Carlos, CA).

Reference

PMID:	19474297
Title:	Functional genomic analysis of amniotic fluid cell-free mRNA suggests that oxidative stress is significant in Down syndrome fetuses
Author:	Slonim DK, Koide K, Johnson KL, Tantravahi U, Cowan JM, Jarrah Z, Bianchi DW
Journal:	Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9425-9.
Description:	To characterize the differences between second trimester Down syndrome (DS) and euploid fetuses, we used Affymetrix microarrays to compare gene expression in uncultured amniotic fluid supernatant samples.