exRNAdisease

General Information

Database ID:

exR0012345

RNA Name:

RPL32

RNA Type:

mRNA

Chromosome:

chr3

Starnd:

Coordinate:

Start Site(bp):

12834485

End Site(bp):

12841582

External Links:

Disease Information

Disease Name:	Down Syndrome
Disease Category:	Congenital, Hereditary, and Neonatal Diseases and Abnormalities
MeSH ID:	D004314
Type:	Diseases Category/Congenital, Hereditary, and Neonatal Diseases and Abnormalities
Alias:	Syndrome, Down//Mongolism//47,XY,+21//Trisomy G//47,XX,+21//Down's Syndrome//Downs Syndrome//Syndrome, Down's//Trisomy 21//Trisomy 21, Mitotic Nondisjunction//Down Syndrome, Partial Trisomy 21//Partial Trisomy 21 Down Syndrome//Trisomy 21, Meiotic Nondisjunction

Expression Detail

GEO ID:	GSE16176
Description:	Expression profiles of amniotic fluid from human fetuses with Down syndrome and euploid controls
Experimental Design:	Disease vs Control
Case Disease Type:	Down Syndrome
Case Disease SubType:	NA
Case Sample:	Down Syndrome
Control Sample:	Normal
Number of Case:	7
Number of Control:	7
Number of Samples:	14

Regulatory Relationship

mRNA targets:

Gene Symbol	Chromosome	Start Site(bp)	End Site(bp)	Strand
ABCB10	chr1	229516582	229558707	-
ABHD4	chr14	22598237	22613215	+
AC004805.1	chr17	65538102	65641033	-
AC010132.3	chr7	42909273	42932174	-
AC023055.1	chr12	55757275	55827546	-
AC073896.1	chr12	56285916	56316059	-
AC104109.3	chr5	134167170	134226071	-
ACO2	chr22	41469117	41528989	+
ACTB	chr7	5527148	5563784	-
ACTR2	chr2	65227753	65271253	+
ADAM10	chr15	58588809	58749791	-
ADD3	chr10	109996368	110135565	+
ADNP	chr20	50888916	50931437	-
AFTPH	chr2	64524305	64593005	+
AGO1	chr1	35869808	35930532	+
AHCY	chr20	34280268	34311802	-
AK3	chr9	4709556	4742043	-
AL645941.2	chr6	32937364	32953122	-
ALCAM	chr3	105366909	105576900	+
ALDOA	chr16	30064164	30070457	+

miRNA targets:

circRNA targets:

lncRNA targets:

lncRNA Symbol	Chromosome	Start Site(bp)	End Site(bp)	Strand
AC007218.1	chr16	9666885	9676843	-
AC019294.2	chr15	75737820	75763322	+
AC021078.1	chr5	149494314	149504670	-
AC026362.1	chr12	122975320	122982907	+
AC087190.3	chr16	9104848	9113181	+
AC087280.2	chr11	6821942	6926878	-
AC117386.2	chr3	150039214	150213726	+
AC124068.2	chr15	89335053	89336161	+
AC245014.3	chr1	145281116	145281462	+
AD000090.1	chr19	35557956	35581954	+

Display:

Experiment Detail

GEO ID:	GSE16176
Sample Source:	Amniotic Fluid
Source Fraction:	Supernatant
Platform:	GPL570
Method:	Microarray
Num of detected RNA Type:	1
Num of detected RNAs of this Type:	17063
Sample treatment protocol:	NA
RNA Extract protocol:	RNA was extracted from 10 mL AF with 30 mL TRIzol LS Reagent (Invitrogen, Carlsbad, CA) and 8 mL chloroform. After RNA extraction, RNA was purified and DNA was removed using the RNeasy® Maxi Kit, including the DNase step according to the manufacturer's protocol (QIAGEN, Valencia, CA). RNA was precipitated using 3M NaOAc and 100% ethanol, and 80% ethanol was added after 4 hours incubation at -20 °C. cDNA, synthesized from extracted RNA, was amplified and purified using the WT-Ovation™ Pico RNA Amplification System (NuGEN, San Carlos, CA) and the DNA Clean & Concentrator™-25 (Zymo Research, Orange, CA).
RNA library preparation protocol:	biotin Samples were labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN, San Carlos, CA).

Reference

PMID:	19474297
Title:	Functional genomic analysis of amniotic fluid cell-free mRNA suggests that oxidative stress is significant in Down syndrome fetuses
Author:	Slonim DK, Koide K, Johnson KL, Tantravahi U, Cowan JM, Jarrah Z, Bianchi DW
Journal:	Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9425-9.
Description:	To characterize the differences between second trimester Down syndrome (DS) and euploid fetuses, we used Affymetrix microarrays to compare gene expression in uncultured amniotic fluid supernatant samples.