exRNAdisease

Entry Detail

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General Information

Database ID: exR0015146 RNA Name: TOB2 RNA Type: mRNA Chromosome: chr22 Starnd: - Coordinate: Start Site(bp): 41433494 End Site(bp): 41446801 External Links: ENSG00000183864

Disease Information

Disease Name: Down Syndrome Disease Category: Congenital, Hereditary, and Neonatal Diseases and Abnormalities MeSH ID: D004314 Type: Diseases Category/Congenital, Hereditary, and Neonatal Diseases and Abnormalities Alias: Syndrome, Down//Mongolism//47,XY,+21//Trisomy G//47,XX,+21//Down's Syndrome//Downs Syndrome//Syndrome, Down's//Trisomy 21//Trisomy 21, Mitotic Nondisjunction//Down Syndrome, Partial Trisomy 21//Partial Trisomy 21 Down Syndrome//Trisomy 21, Meiotic Nondisjunction

Expression Detail

GEO ID: GSE16176 Description: Expression profiles of amniotic fluid from human fetuses with Down syndrome and euploid controls Experimental Design: Disease vs Control Case Disease Type: Down Syndrome Case Disease SubType: NA Case Sample: Down Syndrome Control Sample: Normal Number of Case: 7 Number of Control: 7 Number of Samples: 14

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABL1 chr9 130713016 130887675 + AC004922.1 chr7 99325879 99394653 + AC008878.3 chr19 7382834 7470241 + ACBD6 chr1 180269653 180502954 - ADGRA3 chr4 22345071 22516066 - AHCY chr20 34280268 34311802 - AKAP1 chr17 57085092 57121346 + AL136295.4 chr14 24180395 24190416 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-16-5p chr3 160404754 160404775 + hsa-miR-23a-3p chr19 13836595 13836615 - hsa-miR-103a-3p chr5 168560904 168560926 - hsa-miR-107 chr10 89592756 89592778 - hsa-miR-34a-5p chr1 9151735 9151756 - hsa-miR-23b-3p chr9 95085265 95085287 + hsa-miR-135a-5p chr12 97563834 97563856 + hsa-miR-195-5p chr17 7017667 7017687 - hsa-miR-34c-5p chr11 111513451 111513473 + hsa-miR-378a-3p chr5 149732867 149732888 + hsa-miR-135b-5p chr1 205448361 205448383 - hsa-miR-422a chr15 63870989 63871010 - hsa-miR-424-5p chrX 134546680 134546701 - hsa-miR-432-5p chr14 100884496 100884518 + hsa-miR-512-3p chr19 53666729 53666750 + hsa-miR-520a-5p chr19 53690895 53690915 + hsa-miR-545-3p chrX 74287126 74287147 - hsa-miR-654-5p chr14 101040234 101040255 + hsa-miR-708-5p chr11 79402077 79402099 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC064802.1 chr8 114282067 114295839 + AD000090.1 chr19 35557956 35581954 + AL117378.1 chr6 131901963 131920565 + Display:

Experiment Detail

GEO ID: GSE16176 Sample Source: Amniotic Fluid Source Fraction: Supernatant Platform: GPL570 Method: Microarray Num of detected RNA Type: 1 Num of detected RNAs of this Type: 17063 Sample treatment protocol: NA RNA Extract protocol: RNA was extracted from 10 mL AF with 30 mL TRIzol LS Reagent (Invitrogen, Carlsbad, CA) and 8 mL chloroform. After RNA extraction, RNA was purified and DNA was removed using the RNeasy® Maxi Kit, including the DNase step according to the manufacturer's protocol (QIAGEN, Valencia, CA). RNA was precipitated using 3M NaOAc and 100% ethanol, and 80% ethanol was added after 4 hours incubation at -20 °C. cDNA, synthesized from extracted RNA, was amplified and purified using the WT-Ovation™ Pico RNA Amplification System (NuGEN, San Carlos, CA) and the DNA Clean & Concentrator™-25 (Zymo Research, Orange, CA). RNA library preparation protocol: biotin Samples were labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN, San Carlos, CA).

Reference

PMID: 19474297 Title: Functional genomic analysis of amniotic fluid cell-free mRNA suggests that oxidative stress is significant in Down syndrome fetuses Author: Slonim DK, Koide K, Johnson KL, Tantravahi U, Cowan JM, Jarrah Z, Bianchi DW Journal: Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9425-9. Description: To characterize the differences between second trimester Down syndrome (DS) and euploid fetuses, we used Affymetrix microarrays to compare gene expression in uncultured amniotic fluid supernatant samples.