exRNAdisease

Entry Detail

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General Information

Database ID: exR0226381 RNA Name: hsa-miR-129-5p RNA Type: miRNA Chromosome: chr7 Starnd: + Coordinate: Start Site(bp): 128207876 End Site(bp): 128207896 External Links: hsa-miR-129-5p

Disease Information

Disease Name: Active Tuberculosis Disease Category: Infections MeSH ID: D014376 Type: Diseases Category/Infections Alias: Tuberculosis//Tuberculoses//Kochs Disease//Koch's Disease//Koch Disease//Mycobacterium tuberculosis Infection//Infection, Mycobacterium tuberculosis//Infections, Mycobacterium tuberculosis//Mycobacterium tuberculosis Infections

Expression Detail

GEO ID: GSE124120 Description: Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis Experimental Design: Disease vs Control Case Disease Type: Active Tuberculosis Case Disease SubType: NA Case Sample: Active Tuberculosis Control Sample: Healthy Number of Case: 1 Number of Control: 1 Number of Samples: 2

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand TNRC6B chr22 40044817 40335808 + KRIT1 chr7 92198969 92246166 - SLC7A2 chr8 17497088 17570573 + TRIM71 chr3 32817997 32897824 + SHCBP1 chr16 46578591 46621379 - NEDD4 chr15 55826922 55993746 - ZNF605 chr12 132918306 132956306 - DDX18 chr2 117814691 117832377 + CPSF7 chr11 61402641 61430031 - RAD1 chr5 34905260 34918989 - UBN2 chr7 139230356 139308236 + DEGS1 chr1 224175756 224193441 + SEC62 chr3 169966635 169998373 + CRK chr17 1420689 1463162 - SLC35E1 chr19 16549831 16572415 - TRIM14 chr9 98069275 98119222 - ALDH5A1 chr6 24494867 24537207 + UFL1 chr6 96521595 96555276 + DUSP14 chr17 37489891 37513501 + WSB1 chr17 27294076 27315926 + DNAJC24 chr11 31369840 31432835 + CHURC1 chr14 64914361 64944591 + MLEC chr12 120687149 120701859 + CAMTA1 chr1 6785454 7769706 + THUMPD1 chr16 20702816 20742084 - EIF4A1 chr17 7572706 7579006 + ACER3 chr11 76860867 77026797 + TTPAL chr20 44475874 44494603 + PDS5A chr4 39822863 39977956 - FBXW7 chr4 152320544 152536092 - miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0000943 chr19 47421744 47440665 + hsa_circ_0001168 chr20 47691321 47707559 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC004069.1 chr4 105137280 105140619 - AC097634.1 chr3 71584943 71587409 + AL049795.2 chr1 32170733 32176568 + FGD5-AS1 chr3 14920347 14948424 - MALAT1 chr11 65497688 65506516 + NEAT1 chr11 65422774 65445540 + NUTM2A-AS1 chr10 87201647 87342612 - OIP5-AS1 chr15 41283990 41309737 + TBC1D3P1-DHX40P1 chr17 59962363 60018975 - TUG1 chr22 30969245 30979395 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE124120 Sample Source: Blood Source Fraction: Exosome Platform: GPL16791 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 356 Sample treatment protocol: We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions. RNA Extract protocol: NA RNA library preparation protocol: Small RNA libraries were prepared for sequencing using standard Illumina protocols.

Reference

PMID: https://doi.org/10.3389/fmicb.2019.01174 Title: Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Author: Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang Journal: Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174 Description: In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).