exRNAdisease

Entry Detail

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General Information

Database ID: exR0226389 RNA Name: hsa-miR-148b-3p RNA Type: miRNA Chromosome: chr12 Starnd: + Coordinate: Start Site(bp): 54337278 End Site(bp): 54337299 External Links: hsa-miR-148b-3p

Disease Information

Disease Name: Active Tuberculosis Disease Category: Infections MeSH ID: D014376 Type: Diseases Category/Infections Alias: Tuberculosis//Tuberculoses//Kochs Disease//Koch's Disease//Koch Disease//Mycobacterium tuberculosis Infection//Infection, Mycobacterium tuberculosis//Infections, Mycobacterium tuberculosis//Mycobacterium tuberculosis Infections

Expression Detail

GEO ID: GSE124120 Description: Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis Experimental Design: Disease vs Control Case Disease Type: Active Tuberculosis Case Disease SubType: NA Case Sample: Active Tuberculosis Control Sample: Healthy Number of Case: 1 Number of Control: 1 Number of Samples: 2

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand E2F1 chr20 33675477 33686385 - EMP1 chr12 13196723 13219941 + FAM47E-STBD1 chr4 76251721 76311129 + KLF4 chr9 107484852 107490482 - LENG8 chr19 54448887 54462037 + LYSMD4 chr15 99715697 99733561 - MLPH chr2 237485428 237555322 + MTMR9 chr8 11284816 11328146 + NDST1 chr5 150485818 150558211 + OSBPL11 chr3 125528858 125595497 - PBXIP1 chr1 154944076 154956123 - PFAS chr17 8247618 8270491 + PGRMC1 chrX 119236245 119244466 + RCC1 chr1 28505943 28539300 + RTKN chr2 74425835 74442422 - STARD13 chr13 33103137 33350630 - STBD1 chr4 76306026 76311599 + SYVN1 chr11 65121780 65134533 - TBC1D5 chr3 17157162 18444817 - TMEM123 chr11 102396332 102470384 - TOMM70 chr3 100363431 100401089 - TRIM3 chr11 6448613 6474459 - USP22 chr17 20999596 21043760 - VPS45 chr1 150067279 150145329 + ZNF512B chr20 63956704 63969930 - miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0001454 chr4 154524454 154533552 + hsa_circ_0000384 chr12 27867712 27877119 + hsa_circ_0000284 chr11 33307958 33309057 + hsa_circ_0000943 chr19 47421744 47440665 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC007780.1 chr17 68413623 68524949 + AC073896.4 chr12 56162359 56190284 - AL049840.4 chr14 103694516 103695050 - AL603839.3 chr1 40493157 40508661 - H19 chr11 1995176 2001470 - HCG18 chr6 30286690 30327382 - KCNQ1OT1 chr11 2608328 2699994 - LINC01089 chr12 121795267 121803906 - NEAT1 chr11 65422774 65445540 + NUTM2A-AS1 chr10 87201647 87342612 - NUTM2B-AS1 chr10 79661394 79826594 - XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE124120 Sample Source: Blood Source Fraction: Exosome Platform: GPL16791 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 356 Sample treatment protocol: We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions. RNA Extract protocol: NA RNA library preparation protocol: Small RNA libraries were prepared for sequencing using standard Illumina protocols.

Reference

PMID: https://doi.org/10.3389/fmicb.2019.01174 Title: Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Author: Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang Journal: Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174 Description: In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).