exRNAdisease

Entry Detail

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General Information

Database ID: exR0226399 RNA Name: hsa-miR-199a-3p RNA Type: miRNA Chromosome: chr1 Starnd: - Coordinate: Start Site(bp): 172144554 End Site(bp): 172144575 External Links: hsa-miR-199a-3p

Disease Information

Disease Name: Active Tuberculosis Disease Category: Infections MeSH ID: D014376 Type: Diseases Category/Infections Alias: Tuberculosis//Tuberculoses//Kochs Disease//Koch's Disease//Koch Disease//Mycobacterium tuberculosis Infection//Infection, Mycobacterium tuberculosis//Infections, Mycobacterium tuberculosis//Mycobacterium tuberculosis Infections

Expression Detail

GEO ID: GSE124120 Description: Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis Experimental Design: Disease vs Control Case Disease Type: Active Tuberculosis Case Disease SubType: NA Case Sample: Active Tuberculosis Control Sample: Healthy Number of Case: 1 Number of Control: 1 Number of Samples: 2

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AP3B1 chr5 78000525 78294755 - DUSP5 chr10 110497907 110511533 + E2F3 chr6 20401879 20493714 + EMP1 chr12 13196723 13219941 + PDXDC1 chr16 14974591 15139339 + RBM23 chr14 22893204 22919182 - miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0000591 chr15 41961025 41962156 + hsa_circ_0001731 chr7 100731990 100738448 + hsa_circ_0000311 chr11 61205096 61205585 + hsa_circ_0001865 chr9 86292641 86293514 - lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC005082.1 chr7 23205787 23208045 + AC005165.1 chr7 25593304 25751032 - AC010542.4 chr16 66549280 66551189 + AC021078.1 chr5 149494314 149504670 - AC079781.5 chr7 97851688 97972985 - AL122035.1 chr14 64422935 64448557 - AL358472.3 chr1 153966516 153966930 + MALAT1 chr11 65497688 65506516 + MIR200CHG chr12 6963246 6964447 + NEAT1 chr11 65422774 65445540 + NORAD chr20 36045618 36051018 - OIP5-AS1 chr15 41283990 41309737 + OTUD6B-AS1 chr8 91059318 91070583 - SCARNA9 chr11 93721513 93721865 + SNHG1 chr11 62851984 62855953 - TUG1 chr22 30969245 30979395 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE124120 Sample Source: Blood Source Fraction: Exosome Platform: GPL16791 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 356 Sample treatment protocol: We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions. RNA Extract protocol: NA RNA library preparation protocol: Small RNA libraries were prepared for sequencing using standard Illumina protocols.

Reference

PMID: https://doi.org/10.3389/fmicb.2019.01174 Title: Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Author: Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang Journal: Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174 Description: In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).