exRNAdisease

Entry Detail

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General Information

Database ID: exR0226400 RNA Name: hsa-miR-199a-5p RNA Type: miRNA Chromosome: chr1 Starnd: - Coordinate: Start Site(bp): 172144592 End Site(bp): 172144614 External Links: hsa-miR-199a-5p

Disease Information

Disease Name: Active Tuberculosis Disease Category: Infections MeSH ID: D014376 Type: Diseases Category/Infections Alias: Tuberculosis//Tuberculoses//Kochs Disease//Koch's Disease//Koch Disease//Mycobacterium tuberculosis Infection//Infection, Mycobacterium tuberculosis//Infections, Mycobacterium tuberculosis//Mycobacterium tuberculosis Infections

Expression Detail

GEO ID: GSE124120 Description: Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis Experimental Design: Disease vs Control Case Disease Type: Active Tuberculosis Case Disease SubType: NA Case Sample: Active Tuberculosis Control Sample: Healthy Number of Case: 1 Number of Control: 1 Number of Samples: 2

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ALDH3B2 chr11 67662160 67681224 - CHD2 chr15 92900189 93027996 + COL4A2 chr13 110305812 110513209 + COX6A1 chr12 120438090 120440737 + FAM222B chr17 28755978 28855232 - FOSL2 chr2 28392448 28417317 + HIF1AN chr10 100529072 100559998 + HMGCS1 chr5 43287470 43313512 - IPO7 chr11 9384652 9448127 + KRT80 chr12 52168996 52192014 - MYRF chr11 61752642 61788518 + NFE2L1 chr17 48048329 48061545 + PLPP6 chr9 4662294 4665258 + SLC6A6 chr3 14402576 14489349 + SLC7A5 chr16 87830023 87869507 - WNT5A chr3 55465715 55490539 - XPO6 chr16 28097979 28211920 - ZMIZ1 chr10 79068966 79316528 + miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0000591 chr15 41961025 41962156 + hsa_circ_0001005 chr2 53921020 53978078 - hsa_circ_0000892 chr19 11941431 12014514 + hsa_circ_0000931 chr19 38226804 38231095 - lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC004951.4 chr7 43951910 44019151 - AC095055.1 chr4 151139991 151141103 + BX284668.2 chr1 16870945 16883659 + LINC01783 chr1 16533886 16536172 - POLR2J4 chr7 43940895 44019175 - SNHG12 chr1 28578538 28583132 - TMPO-AS1 chr12 98512973 98516422 - Display:

Experiment Detail

GEO ID: GSE124120 Sample Source: Blood Source Fraction: Exosome Platform: GPL16791 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 356 Sample treatment protocol: We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions. RNA Extract protocol: NA RNA library preparation protocol: Small RNA libraries were prepared for sequencing using standard Illumina protocols.

Reference

PMID: https://doi.org/10.3389/fmicb.2019.01174 Title: Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Author: Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang Journal: Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174 Description: In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).