exRNAdisease

Entry Detail

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General Information

Database ID: exR0226442 RNA Name: hsa-miR-574-5p RNA Type: miRNA Chromosome: chr4 Starnd: + Coordinate: Start Site(bp): 38868056 End Site(bp): 38868078 External Links: hsa-miR-574-5p

Disease Information

Disease Name: Active Tuberculosis Disease Category: Infections MeSH ID: D014376 Type: Diseases Category/Infections Alias: Tuberculosis//Tuberculoses//Kochs Disease//Koch's Disease//Koch Disease//Mycobacterium tuberculosis Infection//Infection, Mycobacterium tuberculosis//Infections, Mycobacterium tuberculosis//Mycobacterium tuberculosis Infections

Expression Detail

GEO ID: GSE124120 Description: Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis Experimental Design: Disease vs Control Case Disease Type: Active Tuberculosis Case Disease SubType: NA Case Sample: Active Tuberculosis Control Sample: Healthy Number of Case: 1 Number of Control: 1 Number of Samples: 2

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand CCDC88C chr14 91271323 91417844 - CARMIL1 chr6 25279078 25620530 + AHCYL1 chr1 109984765 110023742 + ARID3A chr19 925781 975939 + CHD7 chr8 60678740 60868028 + IDH3A chr15 78131498 78171945 + FAM102A chr9 127940582 127980989 - EEF2K chr16 22206278 22288738 + LRP8 chr1 53242364 53328469 - SMCO4 chr11 93478472 93543391 - ALDOA chr16 30064164 30070457 + ZNF621 chr3 40524878 40574685 + MAP7D1 chr1 36155579 36180849 + RAB10 chr2 26034084 26137454 + TACC1 chr8 38728186 38853028 + KLF11 chr2 10042849 10054836 + TEAD1 chr11 12674421 12944737 + MGRN1 chr16 4616493 4690974 + SLC9A1 chr1 27098809 27166981 - FNDC3A chr13 48975912 49209779 + SLC25A43 chrX 119399336 119454478 + CTSD chr11 1752755 1764573 - PHF20L1 chr8 132775358 132848807 + DCAF8 chr1 160215715 160262549 - PIK3R3 chr1 46040140 46133036 - RHOU chr1 228735479 228746664 + GALNT3 chr2 165747588 165794659 - HOXC8 chr12 54008985 54012769 + NID1 chr1 235975830 236065109 - WSB2 chr12 118032694 118062430 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC005394.2 chr19 28435388 28727680 - AC021092.1 chr19 44103007 44113183 - AL358472.3 chr1 153966516 153966930 + DSCAM-AS1 chr21 40383083 40385358 + NEAT1 chr11 65422774 65445540 + SNHG7 chr9 136721366 136728184 - XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE124120 Sample Source: Blood Source Fraction: Exosome Platform: GPL16791 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 356 Sample treatment protocol: We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions. RNA Extract protocol: NA RNA library preparation protocol: Small RNA libraries were prepared for sequencing using standard Illumina protocols.

Reference

PMID: https://doi.org/10.3389/fmicb.2019.01174 Title: Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Author: Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang Journal: Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174 Description: In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).