exRNAdisease

Entry Detail

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General Information

Database ID: exR0226472 RNA Name: hsa-miR-146a-5p RNA Type: miRNA Chromosome: chr5 Starnd: + Coordinate: Start Site(bp): 160485372 End Site(bp): 160485393 External Links: hsa-miR-146a-5p

Disease Information

Disease Name: Latent Tuberculosis Disease Category: Infections MeSH ID: D055985 Type: Diseases Category/Infections Alias: Latent Tuberculoses//Tuberculoses, Latent//Tuberculosis, Latent//Latent Tuberculosis Infection//Infection, Latent Tuberculosis//Infections, Latent Tuberculosis//Latent Tuberculosis Infections//Tuberculosis Infection, Latent//Tuberculosis Infections, Latent

Expression Detail

GEO ID: GSE124120 Description: Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis Experimental Design: Disease vs Control Case Disease Type: Active Tuberculosis Case Disease SubType: NA Case Sample: Latent Tuberculosis Control Sample: Healthy Number of Case: 1 Number of Control: 1 Number of Samples: 2

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ALDOA chr16 30064164 30070457 + ATP6V0D1 chr16 67438014 67481181 - FXYD3 chr19 35115879 35124324 + GATAD1 chr7 92447482 92460075 + HYOU1 chr11 119044188 119057227 - JPT2 chr16 1678256 1702280 + NFIX chr19 12995608 13098796 + RNF169 chr11 74748849 74842413 + SKA2 chr17 59109857 59155260 - SS18 chr18 26016253 26091217 - TACC1 chr8 38728186 38853028 + WDR45B chr17 82614562 82648553 - ZNF512B chr20 63956704 63969930 - ZNRF3 chr22 28883572 29057488 + APEH chr3 49674014 49683971 + DCAF1 chr3 51395867 51500002 - GNAS chr20 58839718 58911192 + HSP90AA1 chr14 102080738 102139699 - KDM1A chr1 23019443 23083689 + MARCKS chr6 113857345 113863475 + NDUFS2 chr1 161197104 161214395 + RBBP7 chrX 16839283 16870414 - RPL9 chr4 39452521 39458949 - SAP18 chr13 21140514 21149084 + SLC25A5 chrX 119468422 119471396 + SPAG5 chr17 28577565 28599025 - miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0000418 chr12 69983264 69985939 + hsa_circ_0001361 chr3 171830241 171851336 + hsa_circ_0000943 chr19 47421744 47440665 + hsa_circ_0000799 chr17 65941524 65972074 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand KCNQ1OT1 chr11 2608328 2699994 - MALAT1 chr11 65497688 65506516 + NEAT1 chr11 65422774 65445540 + SNHG16 chr17 76557764 76565348 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE124120 Sample Source: Blood Source Fraction: Exosome Platform: GPL16791 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 356 Sample treatment protocol: We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions. RNA Extract protocol: NA RNA library preparation protocol: Small RNA libraries were prepared for sequencing using standard Illumina protocols.

Reference

PMID: https://doi.org/10.3389/fmicb.2019.01174 Title: Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Author: Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang Journal: Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174 Description: In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).