Entry Detail


General Information

Database ID:exR0226477
RNA Name:hsa-miR-181a-5p
RNA Type:miRNA
Chromosome:chr1
Starnd:-
Coordinate:
Start Site(bp):198859108End Site(bp):198859130
External Links:hsa-miR-181a-5p



Disease Information

Disease Name:Latent Tuberculosis
Disease Category:Infections
MeSH ID:D055985
Type:Diseases Category/Infections
Alias:Latent Tuberculoses//Tuberculoses, Latent//Tuberculosis, Latent//Latent Tuberculosis Infection//Infection, Latent Tuberculosis//Infections, Latent Tuberculosis//Latent Tuberculosis Infections//Tuberculosis Infection, Latent//Tuberculosis Infections, Latent



Expression Detail

GEO ID:GSE124120
Description:Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis
Experimental Design:Disease vs Control
Case Disease Type:Active Tuberculosis
Case Disease SubType:NA
Case Sample:Latent Tuberculosis
Control Sample:Healthy
Number of Case:1
Number of Control:1
Number of Samples:2





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
CDKN1B
chr12
12715058
12722369
+
ETS1
chr11
128458761
128587558
-
GALNT10
chr5
154190730
154420984
+
IGF2BP3
chr7
23310209
23470491
-
IRS2
chr13
109752695
109786583
-
LAMC1
chr1
183023420
183145592
+
NFIB
chr9
14081843
14398983
-
PPP1R3C
chr10
91628442
91633071
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001550
chr5
167915606
167921655
+
hsa_circ_0000540
chr14
55799534
55821940
+
hsa_circ_0001522
chr5
122881110
122893258
+
hsa_circ_0000892
chr19
11941431
12014514
+
hsa_circ_0001361
chr3
171830241
171851336
+
hsa_circ_0000931
chr19
38226804
38231095
-
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC010442.1
chr5
466124
473098
-
AC010655.4
chr7
128455840
128493859
+
AC021078.1
chr5
149494314
149504670
-
AC144548.1
chr12
110387463
110445548
-
AL035458.2
chr20
34234840
34281173
-
AL049543.1
chr6
28587378
28591747
+
KCNQ1OT1
chr11
2608328
2699994
-
LINC00667
chr18
5237826
5290608
+
MALAT1
chr11
65497688
65506516
+
MIR4458HG
chr5
8450701
8486930
+
NEAT1
chr11
65422774
65445540
+
NORAD
chr20
36045618
36051018
-
OIP5-AS1
chr15
41283990
41309737
+
SNHG1
chr11
62851984
62855953
-
SNHG5
chr6
85650491
85678932
-
SNHG7
chr9
136721366
136728184
-
THUMPD3-AS1
chr3
9349689
9398579
-
XIST
chrX
73820649
73852723
-
ZSCAN16-AS1
chr6
28015122
28137293
-
Display:



Experiment Detail

GEO ID:GSE124120
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL16791
Method:NGS
Num of detected RNA Type:1
Num of detected RNAs of this Type:356
Sample treatment protocol:We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions.
RNA Extract protocol:NA
RNA library preparation protocol:Small RNA libraries were prepared for sequencing using standard Illumina protocols.



Reference

PMID:https://doi.org/10.3389/fmicb.2019.01174
Title:Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis
Author:Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang
Journal:Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174
Description:In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).