exRNAdisease

Entry Detail

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General Information

Database ID: exR0226498 RNA Name: hsa-miR-27b-3p RNA Type: miRNA Chromosome: chr9 Starnd: + Coordinate: Start Site(bp): 95085505 End Site(bp): 95085525 External Links: hsa-miR-27b-3p

Disease Information

Disease Name: Latent Tuberculosis Disease Category: Infections MeSH ID: D055985 Type: Diseases Category/Infections Alias: Latent Tuberculoses//Tuberculoses, Latent//Tuberculosis, Latent//Latent Tuberculosis Infection//Infection, Latent Tuberculosis//Infections, Latent Tuberculosis//Latent Tuberculosis Infections//Tuberculosis Infection, Latent//Tuberculosis Infections, Latent

Expression Detail

GEO ID: GSE124120 Description: Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis Experimental Design: Disease vs Control Case Disease Type: Active Tuberculosis Case Disease SubType: NA Case Sample: Latent Tuberculosis Control Sample: Healthy Number of Case: 1 Number of Control: 1 Number of Samples: 2

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand NUAK1 chr12 106063340 106140033 - IDS chrX 149476988 149521096 - AEN chr15 88621337 88632281 + RTL10 chr22 19846138 19854896 - SOX11 chr2 5692384 5701385 + KCNK1 chr1 233614106 233672514 + ALDH3A2 chr17 19648136 19685760 + GIPC1 chr19 14477760 14496149 - LASP1 chr17 38869859 38921770 + LARP4B chr10 806914 931705 - DCTN4 chr5 150708440 150759109 - TMX4 chr20 7977346 8019805 - B4GALT3 chr1 161171310 161177968 - GLTP chr12 109850945 109880541 - PLK2 chr5 58453982 58460139 - URGCP chr7 43875894 43926411 - ONECUT2 chr18 57435374 57491298 + ACER3 chr11 76860867 77026797 + PRKX chrX 3604340 3713649 - LGALS8 chr1 236518000 236552981 + CDR2 chr16 22345936 22437165 - NRBP1 chr2 27427790 27442259 + CANX chr5 179678628 179731641 + IRF4 chr6 391752 411443 + INO80D chr2 205993721 206086303 - PPP1CC chr12 110719680 110742939 - TTYH3 chr7 2631986 2664802 + IGF2BP3 chr7 23310209 23470491 - KPNA6 chr1 32108056 32176563 + STRBP chr9 123109500 123268586 - miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0000384 chr12 27867712 27877119 + hsa_circ_0001247 chr22 46125304 46136418 + hsa_circ_0000942 chr19 46020920 46021352 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC010980.2 chr2 222317242 222318653 - AC021092.1 chr19 44103007 44113183 - AC022150.4 chr19 52650437 52653284 - AC093010.3 chr3 114314501 114329714 - AL050341.2 chr1 40256427 40257967 - KCNQ1OT1 chr11 2608328 2699994 - LINC01089 chr12 121795267 121803906 - LINC02381 chr12 54126082 54147485 + NEAT1 chr11 65422774 65445540 + SDCBP2-AS1 chr20 1325405 1378735 + SNHG22 chr18 49814023 49851059 + TUG1 chr22 30969245 30979395 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE124120 Sample Source: Blood Source Fraction: Exosome Platform: GPL16791 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 356 Sample treatment protocol: We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions. RNA Extract protocol: NA RNA library preparation protocol: Small RNA libraries were prepared for sequencing using standard Illumina protocols.

Reference

PMID: https://doi.org/10.3389/fmicb.2019.01174 Title: Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Author: Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang Journal: Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174 Description: In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).