exRNAdisease

Entry Detail

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General Information

Database ID: exR0256687 RNA Name: hsa-let-7e-5p RNA Type: miRNA Chromosome: chr19 Starnd: + Coordinate: Start Site(bp): 51692793 End Site(bp): 51692814 External Links: hsa-let-7e-5p

Disease Information

Disease Name: Obesity Disease Category: Other Diseases MeSH ID: D009765 Type: Diseases Category/Nutritional and Metabolic Diseases Alias: Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics

Expression Detail

GEO ID: GSE125494 Description: Adipocyte-Derived Exosomal microRNAs in subject serum samples Experimental Design: Disease vs Control Case Disease Type: Obesity Case Disease SubType: NA Case Sample: Obesity Control Sample: Control Number of Case: 8 Number of Control: 3 Number of Samples: 11

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ARHGAP28 chr18 6729718 6915716 + BACH1 chr21 29194071 29630751 + BCAM chr19 44809071 44821421 + BLOC1S6 chr15 45587214 45615945 + C15orf41 chr15 36579626 36810248 + C19orf53 chr19 13774456 13778773 + COIL chr17 56938199 56961050 - CTPS2 chrX 16587999 16712936 - GRPEL2 chr5 149345430 149354583 + HDLBP chr2 241227264 241317061 - HMGA1 chr6 34236873 34246231 + IGDCC3 chr15 65327127 65378002 - IGF2BP1 chr17 48997385 49056145 + MNT chr17 2384073 2401104 - MPDZ chr9 13105704 13279590 - OPA3 chr19 45527427 45602212 - PACS1 chr11 66070272 66244744 + PDPR chr16 70113626 70162537 + PHC3 chr3 170086732 170181749 - PLD3 chr19 40348456 40380439 + PLPP6 chr9 4662294 4665258 + PPARA chr22 46150521 46243756 + RAB40C chr16 589357 629272 + RANBP2 chr2 108719482 108785809 + SH3PXD2B chr5 172325000 172454525 - TECPR2 chr14 102362941 102502477 + TNRC6B chr22 40044817 40335808 + TRIM65 chr17 75880335 75896951 - UBE2G2 chr21 44768580 44801826 - YOD1 chr1 207043849 207052980 - miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0000247 chr10 74474868 74475660 + hsa_circ_0000987 chr2 30748452 30756180 + hsa_circ_0000246 chr10 74468040 74475660 + hsa_circ_0000018 chr1 15860731 15863309 + hsa_circ_0000038 chr1 28800065 28802803 + hsa_circ_0000799 chr17 65941524 65972074 + hsa_circ_0001164 chr20 45891031 45923523 - lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC006064.5 chr12 6510275 6510522 + AC074117.1 chr2 27356246 27367622 + AC109460.3 chr16 28974804 28990775 + AC124045.1 chr3 44667412 44669364 + AP000766.1 chr11 107312132 107316271 - ARHGAP27P1-BPTFP1-KPNA2P3 chr17 64749663 64781707 - KCNQ1OT1 chr11 2608328 2699994 - LINC00265 chr7 39733430 39793092 + LINC02381 chr12 54126082 54147485 + MIRLET7BHG chr22 46053869 46113928 + NEAT1 chr11 65422774 65445540 + NUTM2A-AS1 chr10 87201647 87342612 - SNHG16 chr17 76557764 76565348 + SNHG4 chr5 139274102 139284899 + TMPO-AS1 chr12 98512973 98516422 - XIST chrX 73820649 73852723 - ZNF436-AS1 chr1 23368997 23371839 + Display:

Experiment Detail

GEO ID: GSE125494 Sample Source: Blood Source Fraction: Exosome Platform: GPL21572 Method: Microarray Num of detected RNA Type: 1 Num of detected RNAs of this Type: 2546 Sample treatment protocol: To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes. RNA Extract protocol: Total RNA was isolated following mirVana's total RNA Isolation kit. RNA library preparation protocol: biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample

Reference

PMID: 31331347 Title: Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs Author: Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ Journal: J Transl Med. 2019 Jul 22;17(1):232. Description: We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.