Entry Detail


General Information

Database ID:exR0256742
RNA Name:hsa-miR-1224-5p
RNA Type:miRNA
Chromosome:chr3
Starnd:+
Coordinate:
Start Site(bp):184241405End Site(bp):184241423
External Links:hsa-miR-1224-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ARF3
chr12
48935723
48957487
-
DHCR7
chr11
71428193
71452868
-
ZNF621
chr3
40524878
40574685
+
SEC14L1
chr17
77088749
77217101
+
EMC10
chr19
50476400
50490870
+
RACGAP1
chr12
49976923
50033136
-
THBD
chr20
23045633
23049672
-
ENSA
chr1
150600851
150629612
-
SLC9A3R1
chr17
74748628
74769353
+
STK4
chr20
44966479
45080021
+
LDOC1
chrX
141111605
141177129
-
UBL5
chr19
9827892
9830115
+
HOXB8
chr17
48611377
48615292
-
ANKRD52
chr12
56237807
56258384
-
TRAM2
chr6
52497408
52577060
-
RNF157
chr17
76142465
76240493
-
STC1
chr8
23841929
23854806
-
LMBR1
chr7
156668946
156893216
-
CALU
chr7
128739292
128773400
+
ATP6V1A
chr3
113747033
113812056
+
KIAA1522
chr1
32741830
32774970
+
IKBKB
chr8
42271302
42332653
+
XPO7
chr8
21919662
22006585
+
MIER3
chr5
56919602
56971675
-
DYRK1B
chr19
39825350
39834201
-
TBC1D20
chr20
435480
462543
-
MAPKAPK2
chr1
206684944
206734283
+
OPA3
chr19
45527427
45602212
-
AK2
chr1
33007940
33080996
-
FYCO1
chr3
45917899
45995824
-
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC009133.5
chr16
29808679
29812227
+
AP000766.1
chr11
107312132
107316271
-
NEAT1
chr11
65422774
65445540
+
TUG1
chr22
30969245
30979395
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.