exRNAdisease

Entry Detail

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General Information

Database ID: exR0256768 RNA Name: hsa-miR-1244 RNA Type: miRNA Chromosome: chr2 Starnd: + Coordinate: Start Site(bp): 231713368 End Site(bp): 231713393 External Links: hsa-miR-1244

Disease Information

Disease Name: Obesity Disease Category: Other Diseases MeSH ID: D009765 Type: Diseases Category/Nutritional and Metabolic Diseases Alias: Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics

Expression Detail

GEO ID: GSE125494 Description: Adipocyte-Derived Exosomal microRNAs in subject serum samples Experimental Design: Disease vs Control Case Disease Type: Obesity Case Disease SubType: NA Case Sample: Obesity Control Sample: Control Number of Case: 8 Number of Control: 3 Number of Samples: 11

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand RHOA chr3 49359145 49412998 - CPNE3 chr8 86514435 86561498 + KIAA1191 chr5 176346062 176361807 - ZMYM4 chr1 35268709 35422058 + CCT2 chr12 69585426 69601570 + AL590764.2 chrX 71103987 71111575 - RPS5 chr19 58386400 58394806 + UBA2 chr19 34428352 34471251 + CDC42EP3 chr2 37641882 37738468 - EEF1G chr11 62559596 62574086 - GAK chr4 849276 932373 - SPIN1 chr9 88388430 88478694 + ERLIN1 chr10 100150094 100186033 - MT-CO3 chrMT 9207 9990 + HNRNPU chr1 244840638 244864560 - TOLLIP chr11 1274371 1309654 - MLF2 chr12 6747996 6767475 - CCDC18 chr1 93179919 93278730 + MBD3 chr19 1573596 1592865 - RPS2 chr16 1962058 1964841 - PTMA chr2 231706895 231713541 + UBC chr12 124911604 124917368 - OAS2 chr12 112978395 113011723 + APELA chr4 164877178 164898965 + SLC7A1 chr13 29509414 29595688 - ARHGDIB chr12 14942031 14961728 - ARMT1 chr6 151452258 151470101 + AP002990.1 chr11 62559603 62591531 - B2M chr15 44711487 44718877 + RFWD3 chr16 74621399 74666877 - miRNA targets: NA circRNA targets: NA lncRNA targets: NA Display:

Experiment Detail

GEO ID: GSE125494 Sample Source: Blood Source Fraction: Exosome Platform: GPL21572 Method: Microarray Num of detected RNA Type: 1 Num of detected RNAs of this Type: 2546 Sample treatment protocol: To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes. RNA Extract protocol: Total RNA was isolated following mirVana's total RNA Isolation kit. RNA library preparation protocol: biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample

Reference

PMID: 31331347 Title: Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs Author: Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ Journal: J Transl Med. 2019 Jul 22;17(1):232. Description: We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.