Entry Detail


General Information

Database ID:exR0256775
RNA Name:hsa-miR-1248
RNA Type:miRNA
Chromosome:chr3
Starnd:+
Coordinate:
Start Site(bp):186786675End Site(bp):186786701
External Links:hsa-miR-1248



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
CHD9
chr16
53055033
53329150
+
ADAM10
chr15
58588809
58749791
-
COA1
chr7
43608456
43729717
-
COMT
chr22
19941733
19969975
+
COPB1
chr11
14443440
14500027
-
EIF3L
chr22
37848868
37889407
+
EIF4A2
chr3
186783205
186789897
+
GSPT1
chr16
11868128
11916082
-
HNRNPA0
chr5
137745651
137754363
-
INO80B
chr2
74455087
74457944
+
INO80B-WBP1
chr2
74455088
74460884
+
KCTD10
chr12
109448655
109477359
-
MRPL13
chr8
120380761
120445402
-
RPS25
chr11
119015712
119018691
-
SEC24B
chr4
109433772
109540896
+
SERINC5
chr5
80111651
80256048
-
TM9SF2
chr13
99446311
99564048
+
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
SNHG1
chr11
62851984
62855953
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.