Entry Detail


General Information

Database ID:exR0256794
RNA Name:hsa-miR-125b-5p
RNA Type:miRNA
Chromosome:chr21
Starnd:+
Coordinate:
Start Site(bp):16590253End Site(bp):16590274
External Links:hsa-miR-125b-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
RYBP
chr3
72371825
72446621
-
TRIM65
chr17
75880335
75896951
-
TYSND1
chr10
70137981
70146700
-
UGGT1
chr2
128091200
128195677
+
ZBTB39
chr12
56998836
57006546
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000370
chr11
128628009
128651918
+
hsa_circ_0001723
chr7
91924202
91948826
+
hsa_circ_0001492
chr5
65284462
65290692
+
hsa_circ_0000494
chr13
76134888
76143643
+
hsa_circ_0001307
chr3
51575513
51624575
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC007780.1
chr17
68413623
68524949
+
AC022167.2
chr16
8848105
8860456
+
AC084082.1
chr8
66112667
66126632
+
AC108134.2
chr16
3156736
3157483
-
AC245014.3
chr1
145281116
145281462
+
AL049840.4
chr14
103694516
103695050
-
AL132780.1
chr14
22929607
22956374
+
AL137782.1
chr13
75549773
75807120
+
AL162258.1
chr1
153626332
153634340
-
DANCR
chr4
52712404
52720351
+
KCNQ1OT1
chr11
2608328
2699994
-
NEAT1
chr11
65422774
65445540
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.