Entry Detail


General Information

Database ID:exR0256796
RNA Name:hsa-miR-126-5p
RNA Type:miRNA
Chromosome:chr9
Starnd:+
Coordinate:
Start Site(bp):136670616End Site(bp):136670636
External Links:hsa-miR-126-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:NA
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001731
chr7
100731990
100738448
+
hsa_circ_0000598
chr15
45009906
45009989
+
hsa_circ_0000943
chr19
47421744
47440665
+
hsa_circ_0001146
chr20
34241449
34246936
-
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC009032.1
chr16
69632141
69632571
+
AC010655.4
chr7
128455840
128493859
+
AC078846.1
chr7
129604548
129611630
-
AC124798.1
chr11
17380649
17383531
+
FGD5-AS1
chr3
14920347
14948424
-
HOTAIR
chr12
53962308
53974956
-
LINC00667
chr18
5237826
5290608
+
LINC00943
chr12
126723412
126749027
+
MALAT1
chr11
65497688
65506516
+
NEAT1
chr11
65422774
65445540
+
NUTM2B-AS1
chr10
79661394
79826594
-
OIP5-AS1
chr15
41283990
41309737
+
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.