Entry Detail


General Information

Database ID:exR0256823
RNA Name:hsa-miR-1277-5p
RNA Type:miRNA
Chromosome:chrX
Starnd:+
Coordinate:
Start Site(bp):118386401End Site(bp):118386424
External Links:hsa-miR-1277-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:NA
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000417
chr12
69644908
69656342
+
hsa_circ_0001168
chr20
47691321
47707559
+
hsa_circ_0000552
chr14
71880664
71948928
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC005034.3
chr2
75697583
75697996
+
AC005082.1
chr7
23205787
23208045
+
AC010327.5
chr19
55216660
55221616
+
AC024940.6
chr12
31280422
31280895
-
AC079781.5
chr7
97851688
97972985
-
AL050341.2
chr1
40256427
40257967
-
DANCR
chr4
52712404
52720351
+
EPB41L4A-AS1
chr5
112160526
112164818
+
HCG11
chr6
26521709
26527404
+
HOTAIR
chr12
53962308
53974956
-
NEAT1
chr11
65422774
65445540
+
NORAD
chr20
36045618
36051018
-
OIP5-AS1
chr15
41283990
41309737
+
PSMA3-AS1
chr14
58265365
58298134
-
SH3BP5-AS1
chr3
15254184
15264515
+
TBC1D3P1-DHX40P1
chr17
59962363
60018975
-
TUG1
chr22
30969245
30979395
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.