Entry Detail


General Information

Database ID:exR0256828
RNA Name:hsa-miR-128-3p
RNA Type:miRNA
Chromosome:chr2
Starnd:+
Coordinate:
Start Site(bp):135665446End Site(bp):135665466
External Links:hsa-miR-128-3p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:NA
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000384
chr12
27867712
27877119
+
hsa_circ_0000942
chr19
46020920
46021352
+
hsa_circ_0001865
chr9
86292641
86293514
-
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC012181.2
chr16
56940278
56941342
+
AC021078.1
chr5
149494314
149504670
-
AC021092.1
chr19
44103007
44113183
-
AC239868.1
chr1
149861271
149862504
+
ARHGAP27P1-BPTFP1-KPNA2P3
chr17
64749663
64781707
-
CYTOR
chr2
87454781
87636740
+
KCNQ1OT1
chr11
2608328
2699994
-
MIR4435-2HG
chr2
111006015
111523376
-
MIR497HG
chr17
7015818
7019659
-
NEAT1
chr11
65422774
65445540
+
OIP5-AS1
chr15
41283990
41309737
+
SNHG16
chr17
76557764
76565348
+
SNHG22
chr18
49814023
49851059
+
TUG1
chr22
30969245
30979395
+
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.