Entry Detail


General Information

Database ID:exR0256900
RNA Name:hsa-miR-140-5p
RNA Type:miRNA
Chromosome:chr16
Starnd:+
Coordinate:
Start Site(bp):69933103End Site(bp):69933124
External Links:hsa-miR-140-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ARIH1
chr15
72474330
72602987
+
BTBD7
chr14
93237550
93333092
-
C15orf41
chr15
36579626
36810248
+
CLPTM1
chr19
44954585
44993341
+
COL4A2
chr13
110305812
110513209
+
ITPRIPL2
chr16
19113932
19121629
+
NME4
chr16
396725
410367
+
POLR3G
chr5
90471748
90514557
+
SCRN1
chr7
29920104
29990289
-
SLC6A6
chr3
14402576
14489349
+
SRP14
chr15
40035690
40039181
-
TMEM181
chr6
158536436
158635428
+
TNRC6B
chr22
40044817
40335808
+
TRIO
chr5
14143342
14532128
+
ZNF800
chr7
127346790
127431924
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000712
chr16
68208276
68225678
+
hsa_circ_0001699
chr7
40027197
40041630
+
hsa_circ_0001472
chr5
36953719
36976504
+
hsa_circ_0000032
chr1
25666964
25669564
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AL162258.1
chr1
153626332
153634340
-
DHRS4-AS1
chr14
23938219
24052555
-
H19
chr11
1995176
2001470
-
KCNQ1OT1
chr11
2608328
2699994
-
MALAT1
chr11
65497688
65506516
+
NEAT1
chr11
65422774
65445540
+
SNHG1
chr11
62851984
62855953
-
SNHG16
chr17
76557764
76565348
+
SNHG20
chr17
77086716
77099902
+
TDRKH-AS1
chr1
151790804
151794402
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.