Entry Detail


General Information

Database ID:exR0256910
RNA Name:hsa-miR-145-5p
RNA Type:miRNA
Chromosome:chr5
Starnd:+
Coordinate:
Start Site(bp):149430661End Site(bp):149430683
External Links:hsa-miR-145-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ACBD5
chr10
27195214
27242130
-
ATP9A
chr20
51596514
51768390
-
BAZ1B
chr7
73440406
73522293
-
CARMIL1
chr6
25279078
25620530
+
CCNJ
chr10
96043394
96060870
+
MYRF
chr11
61752642
61788518
+
PLEKHM1
chr17
45435900
45490749
-
PPP1R9B
chr17
50133737
50150677
-
RAD23B
chr9
107283137
107332192
+
ZNF28
chr19
52797408
52857600
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001454
chr4
154524454
154533552
+
hsa_circ_0000540
chr14
55799534
55821940
+
hsa_circ_0001731
chr7
100731990
100738448
+
hsa_circ_0001492
chr5
65284462
65290692
+
hsa_circ_0001414
chr4
56277780
56284152
+
hsa_circ_0000943
chr19
47421744
47440665
+
hsa_circ_0001168
chr20
47691321
47707559
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC006064.5
chr12
6510275
6510522
+
AC016876.2
chr17
7581964
7584086
-
AC023509.1
chr12
53441741
53467528
+
AC105339.2
chr15
82752884
82757208
+
AC144548.1
chr12
110387463
110445548
-
AL031282.2
chr1
1702736
1737688
-
JPX
chrX
73944182
74070408
+
KCNQ1OT1
chr11
2608328
2699994
-
LINC00852
chr3
10284419
10285746
+
MALAT1
chr11
65497688
65506516
+
MEG3
chr14
100779410
100861031
+
SNHG1
chr11
62851984
62855953
-
TUG1
chr22
30969245
30979395
+
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.