Entry Detail


General Information

Database ID:exR0256965
RNA Name:hsa-miR-182-5p
RNA Type:miRNA
Chromosome:chr7
Starnd:-
Coordinate:
Start Site(bp):129770447End Site(bp):129770470
External Links:hsa-miR-182-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
BCL11A
chr2
60450520
60554467
-
CTDSP2
chr12
57819927
57846729
-
EVI5
chr1
92508696
92792404
-
KHSRP
chr19
6413348
6424794
-
PAN2
chr12
56316223
56334053
-
PKN2
chr1
88684222
88836255
+
PLXNA1
chr3
126988594
127037392
+
PRLR
chr5
35048756
35230487
-
RPS29
chr14
49570984
49599164
-
SDCBP
chr8
58552924
58582859
+
SLC30A1
chr1
211571568
211579161
-
ZNF189
chr9
101398873
101410660
+
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001728
chr7
99695763
99695845
-
hsa_circ_0001409
chr4
48835417
48859382
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC005034.3
chr2
75697583
75697996
+
AC018521.1
chr17
47945424
47981736
+
AC091564.7
chr11
6610883
6616594
-
AL137129.1
chr14
61734138
61776260
+
HAGLR
chr2
176164051
176188958
-
KCNQ1OT1
chr11
2608328
2699994
-
LAMTOR5-AS1
chr1
110347116
110443817
+
NEAT1
chr11
65422774
65445540
+
NORAD
chr20
36045618
36051018
-
SNHG1
chr11
62851984
62855953
-
SNHG16
chr17
76557764
76565348
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.