Entry Detail


General Information

Database ID:exR0256974
RNA Name:hsa-miR-186-5p
RNA Type:miRNA
Chromosome:chr1
Starnd:-
Coordinate:
Start Site(bp):71067681End Site(bp):71067702
External Links:hsa-miR-186-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
DDX3X
chrX
41333284
41364472
+
EP300
chr22
41092592
41180077
+
GPRC5A
chr12
12890782
12917937
+
NRF1
chr7
129611720
129757082
+
TACSTD2
chr1
58575433
58577252
-
TMEM245
chr9
109015135
109119947
-
TNFRSF21
chr6
47231532
47309905
-
TNKS1BP1
chr11
57299638
57324952
-
HMG20A
chr15
77420412
77485607
+
RNF139
chr8
124474738
124487914
+
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000892
chr19
11941431
12014514
+
hsa_circ_0000799
chr17
65941524
65972074
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC021078.1
chr5
149494314
149504670
-
AC097634.1
chr3
71584943
71587409
+
AC124798.1
chr11
17380649
17383531
+
AC247036.1
chr14
106206603
106208571
+
DLEU2
chr13
49956670
50125720
-
DSCAM-AS1
chr21
40383083
40385358
+
EBLN3P
chr9
37079645
37090928
+
NEAT1
chr11
65422774
65445540
+
OIP5-AS1
chr15
41283990
41309737
+
SNHG3
chr1
28505980
28510892
+
SNHG7
chr9
136721366
136728184
-
TUG1
chr22
30969245
30979395
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.