Entry Detail


General Information

Database ID:exR0257024
RNA Name:hsa-miR-199b-5p
RNA Type:miRNA
Chromosome:chr9
Starnd:-
Coordinate:
Start Site(bp):128244783End Site(bp):128244805
External Links:hsa-miR-199b-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ALDH3B2
chr11
67662160
67681224
-
ATP9A
chr20
51596514
51768390
-
CHD2
chr15
92900189
93027996
+
COL4A2
chr13
110305812
110513209
+
COX6A1
chr12
120438090
120440737
+
FAM222B
chr17
28755978
28855232
-
FOSL2
chr2
28392448
28417317
+
HMGCS1
chr5
43287470
43313512
-
KRT80
chr12
52168996
52192014
-
MYRF
chr11
61752642
61788518
+
NFE2L1
chr17
48048329
48061545
+
SLC7A5
chr16
87830023
87869507
-
WNT5A
chr3
55465715
55490539
-
XPO6
chr16
28097979
28211920
-
ZMIZ1
chr10
79068966
79316528
+
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000591
chr15
41961025
41962156
+
hsa_circ_0001005
chr2
53921020
53978078
-
hsa_circ_0000892
chr19
11941431
12014514
+
hsa_circ_0000931
chr19
38226804
38231095
-
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC004951.4
chr7
43951910
44019151
-
AC095055.1
chr4
151139991
151141103
+
BX284668.2
chr1
16870945
16883659
+
LINC01783
chr1
16533886
16536172
-
POLR2J4
chr7
43940895
44019175
-
SNHG12
chr1
28578538
28583132
-
TMPO-AS1
chr12
98512973
98516422
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.