Entry Detail


General Information

Database ID:exR0257030
RNA Name:hsa-miR-200a-3p
RNA Type:miRNA
Chromosome:chr1
Starnd:+
Coordinate:
Start Site(bp):1167916End Site(bp):1167937
External Links:hsa-miR-200a-3p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ANP32E
chr1
150218417
150236156
-
ARPC5
chr1
183620846
183635783
-
GALNT10
chr5
154190730
154420984
+
KRT10
chr17
40818117
40822614
-
PDHA1
chrX
19343893
19361718
+
PIGW
chr17
36534987
36539310
+
QKI
chr6
163414000
163578592
+
QSER1
chr11
32892820
32993316
+
RMND5A
chr2
86720291
86778041
+
TNRC6B
chr22
40044817
40335808
+
C6orf62
chr6
24704861
24719998
-
PLAG1
chr8
56160909
56211324
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001329
chr3
119219541
119222868
+
hsa_circ_0001376
chr3
194169185
194175121
-
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC016831.1
chr7
130876809
130913310
-
AC023509.1
chr12
53441741
53467528
+
AC234582.1
chr1
155195004
155205495
+
AL049840.4
chr14
103694516
103695050
-
MALAT1
chr11
65497688
65506516
+
MIR29B2CHG
chr1
207801518
207879115
-
NEAT1
chr11
65422774
65445540
+
OIP5-AS1
chr15
41283990
41309737
+
PINK1-AS
chr1
20642657
20652193
-
SNHG15
chr7
44983023
44986961
-
SNHG16
chr17
76557764
76565348
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.