exRNAdisease

Entry Detail

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General Information

Database ID: exR0257034 RNA Name: hsa-miR-200c-3p RNA Type: miRNA Chromosome: chr12 Starnd: + Coordinate: Start Site(bp): 6963742 End Site(bp): 6963764 External Links: hsa-miR-200c-3p

Disease Information

Disease Name: Obesity Disease Category: Other Diseases MeSH ID: D009765 Type: Diseases Category/Nutritional and Metabolic Diseases Alias: Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics

Expression Detail

GEO ID: GSE125494 Description: Adipocyte-Derived Exosomal microRNAs in subject serum samples Experimental Design: Disease vs Control Case Disease Type: Obesity Case Disease SubType: NA Case Sample: Obesity Control Sample: Control Number of Case: 8 Number of Control: 3 Number of Samples: 11

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand CHD9 chr16 53055033 53329150 + CTNND1 chr11 57753243 57819546 + FOXP1 chr3 70952817 71583993 - GPR180 chr13 94601857 94634661 + KLF3 chr4 38664197 38701517 + NRBP1 chr2 27427790 27442259 + NRIP1 chr21 14961235 15065936 - PTP4A1 chr6 63521746 63583436 + RBM12 chr20 35648925 35664956 - SLC35B4 chr7 134289332 134316930 - SMAD7 chr18 48919853 48952052 - SRP72 chr4 56467617 56503681 + THAP2 chr12 71664301 71680644 + XPO4 chr13 20777329 20903048 - AC008982.1 chr19 38817471 38840178 - CASD1 chr7 94509219 94557019 + CEBPZ chr2 37201612 37231596 - ECH1 chr19 38815422 38831841 - PSMB2 chr1 35599541 35641526 - SNX5 chr20 17941597 17968980 - SZRD1 chr1 16352575 16398145 + WDR13 chrX 48590042 48608869 + ZNF217 chr20 53567065 53609907 - miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0001731 chr7 100731990 100738448 + hsa_circ_0001486 chr5 56526672 56527148 + hsa_circ_0000552 chr14 71880664 71948928 + hsa_circ_0001358 chr3 169694733 169706147 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC024940.6 chr12 31280422 31280895 - AC092295.2 chr19 36489649 36491040 + AC116913.1 chr15 66488658 66492109 - AF254983.1 chr21 10482738 10605716 + AL049840.4 chr14 103694516 103695050 - LINC00667 chr18 5237826 5290608 + MALAT1 chr11 65497688 65506516 + NEAT1 chr11 65422774 65445540 + OIP5-AS1 chr15 41283990 41309737 + SNHG22 chr18 49814023 49851059 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE125494 Sample Source: Blood Source Fraction: Exosome Platform: GPL21572 Method: Microarray Num of detected RNA Type: 1 Num of detected RNAs of this Type: 2546 Sample treatment protocol: To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes. RNA Extract protocol: Total RNA was isolated following mirVana's total RNA Isolation kit. RNA library preparation protocol: biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample

Reference

PMID: 31331347 Title: Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs Author: Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ Journal: J Transl Med. 2019 Jul 22;17(1):232. Description: We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.