Entry Detail


General Information

Database ID:exR0257082
RNA Name:hsa-miR-217-5p
RNA Type:miRNA
Chromosome:chr2
Starnd:-
Coordinate:
Start Site(bp):55983020End Site(bp):55983042
External Links:hsa-miR-217-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
PNPO
chr17
47941506
47949308
+
SLC26A2
chr5
149960758
149993455
+
ICAM1
chr19
10271093
10286615
+
ARHGAP11A
chr15
32615144
32639941
+
SFXN1
chr5
175477062
175529742
+
EIF1AD
chr11
65996545
66002176
-
KPNA4
chr3
160495007
160565571
-
LYSMD4
chr15
99715697
99733561
-
AXL
chr19
41219223
41261766
+
OPA3
chr19
45527427
45602212
-
IRF4
chr6
391752
411443
+
SLC25A32
chr8
103398635
103415189
-
UBE2G1
chr17
4269259
4366628
-
MMD
chr17
55392622
55421924
-
CDH1
chr16
68737292
68835541
+
ICK
chr6
53001279
53061802
-
PARD6B
chr20
50731580
50756795
+
GOLGA3
chr12
132768914
132829078
-
MET
chr7
116672196
116798386
+
ACER2
chr9
19409009
19452505
+
PSKH1
chr16
67893254
67929676
+
EIF2S3
chrX
24054946
24078810
+
VHL
chr3
10141008
10152220
+
DDX3X
chrX
41333284
41364472
+
ZNF587
chr19
57849859
57865117
+
TSEN54
chr17
75516060
75524735
+
CNN2
chr19
1026586
1039068
+
CHD9
chr16
53055033
53329150
+
ERLIN1
chr10
100150094
100186033
-
TFPI
chr2
187464230
187565760
-
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
ADAMTSL4-AS1
chr1
150560202
150574552
-
AP000553.1
chr22
21652270
21670237
+
GAS5
chr1
173858559
173868882
-
LINC01578
chr15
92819540
92899701
+
MALAT1
chr11
65497688
65506516
+
NEAT1
chr11
65422774
65445540
+
OIP5-AS1
chr15
41283990
41309737
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.