Entry Detail


General Information

Database ID:exR0257085
RNA Name:hsa-miR-218-5p
RNA Type:miRNA
Chromosome:chr5
Starnd:-
Coordinate:
Start Site(bp):168768211End Site(bp):168768231
External Links:hsa-miR-218-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ABI2
chr2
203328239
203447728
+
ADCY9
chr16
3953387
4116442
-
CPA4
chr7
130293134
130324180
+
GRK2
chr11
67266473
67286556
+
IGF2
chr11
2129112
2141238
-
LCLAT1
chr2
30447226
30644225
+
LIN28B
chr6
104936616
105083332
+
OXSR1
chr3
38165089
38255484
+
PARD6B
chr20
50731580
50756795
+
PRRC1
chr5
127517609
127555089
+
RBBP9
chr20
18486540
18497225
-
THUMPD1
chr16
20702816
20742084
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000798
chr17
65941524
65944422
+
hsa_circ_0000155
chr1
168007608
168014465
+
hsa_circ_0001452
chr4
153562079
153574079
-
hsa_circ_0001860
chr9
37126308
37126939
+
hsa_circ_0001146
chr20
34241449
34246936
-
hsa_circ_0000799
chr17
65941524
65972074
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC004069.1
chr4
105137280
105140619
-
AC021092.1
chr19
44103007
44113183
-
AC125257.1
chr17
41848518
41851447
-
ADAMTSL4-AS1
chr1
150560202
150574552
-
KCNQ1OT1
chr11
2608328
2699994
-
NEAT1
chr11
65422774
65445540
+
OIP5-AS1
chr15
41283990
41309737
+
PSMA3-AS1
chr14
58265365
58298134
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.