Entry Detail


General Information

Database ID:exR0257095
RNA Name:hsa-miR-222-3p
RNA Type:miRNA
Chromosome:chrX
Starnd:-
Coordinate:
Start Site(bp):45747036End Site(bp):45747056
External Links:hsa-miR-222-3p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
CRKL
chr22
20917407
20953747
+
DNAJC14
chr12
55820960
55830824
-
FOXP1
chr3
70952817
71583993
-
KDSR
chr18
63327726
63367228
-
LRP6
chr12
12116025
12267044
-
MPZL1
chr1
167721192
167791919
+
NFIB
chr9
14081843
14398983
-
PALLD
chr4
168497052
168928457
+
TNPO1
chr5
72816312
72916733
+
TUB
chr11
8019244
8106112
+
NME7
chr1
169132531
169367948
-
PRPF40A
chr2
152651593
152717997
-
RIF1
chr2
151409883
151508013
+
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001247
chr22
46125304
46136418
+
hsa_circ_0000670
chr16
9199043
9199243
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC020978.7
chr16
68224713
68227734
+
AC022150.4
chr19
52650437
52653284
-
ARHGAP27P1-BPTFP1-KPNA2P3
chr17
64749663
64781707
-
GAS5
chr1
173858559
173868882
-
NEAT1
chr11
65422774
65445540
+
TUG1
chr22
30969245
30979395
+
TYMSOS
chr18
630886
658340
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.