Entry Detail


General Information

Database ID:exR0257132
RNA Name:hsa-miR-27b-3p
RNA Type:miRNA
Chromosome:chr9
Starnd:+
Coordinate:
Start Site(bp):95085505End Site(bp):95085525
External Links:hsa-miR-27b-3p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
NUAK1
chr12
106063340
106140033
-
IDS
chrX
149476988
149521096
-
AEN
chr15
88621337
88632281
+
RTL10
chr22
19846138
19854896
-
SOX11
chr2
5692384
5701385
+
KCNK1
chr1
233614106
233672514
+
ALDH3A2
chr17
19648136
19685760
+
GIPC1
chr19
14477760
14496149
-
LASP1
chr17
38869859
38921770
+
LARP4B
chr10
806914
931705
-
DCTN4
chr5
150708440
150759109
-
TMX4
chr20
7977346
8019805
-
B4GALT3
chr1
161171310
161177968
-
GLTP
chr12
109850945
109880541
-
PLK2
chr5
58453982
58460139
-
URGCP
chr7
43875894
43926411
-
ONECUT2
chr18
57435374
57491298
+
ACER3
chr11
76860867
77026797
+
PRKX
chrX
3604340
3713649
-
LGALS8
chr1
236518000
236552981
+
CDR2
chr16
22345936
22437165
-
NRBP1
chr2
27427790
27442259
+
CANX
chr5
179678628
179731641
+
IRF4
chr6
391752
411443
+
INO80D
chr2
205993721
206086303
-
PPP1CC
chr12
110719680
110742939
-
TTYH3
chr7
2631986
2664802
+
IGF2BP3
chr7
23310209
23470491
-
KPNA6
chr1
32108056
32176563
+
STRBP
chr9
123109500
123268586
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000384
chr12
27867712
27877119
+
hsa_circ_0001247
chr22
46125304
46136418
+
hsa_circ_0000942
chr19
46020920
46021352
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC010980.2
chr2
222317242
222318653
-
AC021092.1
chr19
44103007
44113183
-
AC022150.4
chr19
52650437
52653284
-
AC093010.3
chr3
114314501
114329714
-
AL050341.2
chr1
40256427
40257967
-
KCNQ1OT1
chr11
2608328
2699994
-
LINC01089
chr12
121795267
121803906
-
LINC02381
chr12
54126082
54147485
+
NEAT1
chr11
65422774
65445540
+
SDCBP2-AS1
chr20
1325405
1378735
+
SNHG22
chr18
49814023
49851059
+
TUG1
chr22
30969245
30979395
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.