Entry Detail


General Information

Database ID:exR0257138
RNA Name:hsa-miR-296-3p
RNA Type:miRNA
Chromosome:chr20
Starnd:-
Coordinate:
Start Site(bp):58817626End Site(bp):58817647
External Links:hsa-miR-296-3p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ATXN7
chr3
63864557
64003462
+
BAZ2A
chr12
56595596
56636816
-
COL6A1
chr21
45981769
46005050
+
CYLD
chr16
50742050
50801935
+
FAM210A
chr18
13663347
13726663
-
HMGCS1
chr5
43287470
43313512
-
HOXC10
chr12
53985065
53990279
+
HP1BP3
chr1
20740266
20787323
-
KAT6A
chr8
41929479
42051994
-
KMT2D
chr12
49018975
49059774
-
KPNA6
chr1
32108056
32176563
+
LASP1
chr17
38869859
38921770
+
MINK1
chr17
4833340
4898061
+
NFAT5
chr16
69565094
69704666
+
PFN2
chr3
149964904
150050788
-
RTKN
chr2
74425835
74442422
-
SERF2
chr15
43777087
43802589
+
SLC2A4RG
chr20
63739776
63744050
+
STK35
chr20
2101827
2177038
+
TMEM97
chr17
28319200
28328685
+
TP53
chr17
7661779
7687550
-
TULP3
chr12
2877223
2941138
+
UBA2
chr19
34428352
34471251
+
VANGL1
chr1
115641970
115698224
+
ZFP91
chr11
58579063
58621550
+
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001867
chr9
86294689
86297981
-
hsa_circ_0001866
chr9
86293355
86297981
-
hsa_circ_0001046
chr2
97024782
97033111
+
hsa_circ_0001146
chr20
34241449
34246936
-
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC009133.5
chr16
29808679
29812227
+
AC016831.1
chr7
130876809
130913310
-
AC023509.1
chr12
53441741
53467528
+
NEAT1
chr11
65422774
65445540
+
NORAD
chr20
36045618
36051018
-
PCBP2-OT1
chr12
53464468
53465057
+
SNHG25
chr17
64145970
64146476
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.