Entry Detail


General Information

Database ID:exR0257139
RNA Name:hsa-miR-296-5p
RNA Type:miRNA
Chromosome:chr20
Starnd:-
Coordinate:
Start Site(bp):58817661End Site(bp):58817681
External Links:hsa-miR-296-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ACTN4
chr19
38647649
38731589
+
CBX1
chr17
48070052
48101478
-
EPHB4
chr7
100802565
100827523
-
FOXP4
chr6
41546426
41602384
+
GOSR2
chr17
46923075
46975524
+
RFX1
chr19
13961530
14007039
-
WDR5
chr9
134135365
134159968
+
ZCCHC3
chr20
297570
300321
+
GNL1
chr6
30541377
30557174
-
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC016876.2
chr17
7581964
7584086
-
AL132655.2
chr20
58817132
58817725
-
AL355075.4
chr14
20343048
20343685
-
AL356488.2
chr1
109100193
109100619
+
KCNQ1OT1
chr11
2608328
2699994
-
MIR34AHG
chr1
9148011
9198906
-
MIR663AHG
chr20
26167817
26251546
-
NEAT1
chr11
65422774
65445540
+
NORAD
chr20
36045618
36051018
-
STAG3L5P-PVRIG2P-PILRB
chr7
100336104
100367831
+
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.