Entry Detail


General Information

Database ID:exR0257163
RNA Name:hsa-miR-302e
RNA Type:miRNA
Chromosome:chr11
Starnd:+
Coordinate:
Start Site(bp):7234771End Site(bp):7234787
External Links:hsa-miR-302e



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ATP6V1B2
chr8
20197381
20226819
+
BTN3A1
chr6
26402237
26415208
+
CCSAP
chr1
229321011
229343294
-
CELF2
chr10
10798397
11336675
+
CLCN6
chr1
11806096
11848079
+
COL4A2
chr13
110305812
110513209
+
CUL1
chr7
148697914
148801110
+
KMT5B
chr11
68154863
68213828
-
MTMR12
chr5
32226994
32312987
-
OCRL
chrX
129539849
129592561
+
PFN2
chr3
149964904
150050788
-
PLAGL2
chr20
32192504
32207743
-
PLEKHM1
chr17
45435900
45490749
-
SEC16A
chr9
136440096
136483759
-
SLC7A2
chr8
17497088
17570573
+
SYAP1
chrX
16719612
16765340
+
TARDBP
chr1
11012344
11026420
+
TRIB1
chr8
125430358
125438403
+
TXNIP
chr1
145992435
145996579
-
WASF3
chr13
26557683
26688948
+
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000943
chr19
47421744
47440665
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AL049840.4
chr14
103694516
103695050
-
ARHGAP27P1-BPTFP1-KPNA2P3
chr17
64749663
64781707
-
FGD5-AS1
chr3
14920347
14948424
-
MIR17HG
chr13
91347820
91354579
+
NEAT1
chr11
65422774
65445540
+
SNHG16
chr17
76557764
76565348
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.