Entry Detail


General Information

Database ID:exR0257319
RNA Name:hsa-miR-324-3p
RNA Type:miRNA
Chromosome:chr17
Starnd:-
Coordinate:
Start Site(bp):7223308End Site(bp):7223330
External Links:hsa-miR-324-3p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ACLY
chr17
41866917
41930542
-
ATXN7L3
chr17
44191805
44200113
-
CDC42EP2
chr11
65314866
65322417
+
CELSR2
chr1
109249539
109275751
+
DBNDD1
chr16
90004871
90020128
-
DCAF15
chr19
13952492
13961449
+
FBXL19
chr16
30923055
30948783
+
LNPEP
chr5
96935394
97037513
+
MAPKAPK2
chr1
206684944
206734283
+
MXD4
chr4
2247432
2262109
-
NACC1
chr19
13116862
13141147
+
PDRG1
chr20
31944337
31952046
-
SMAD7
chr18
48919853
48952052
-
SPTBN2
chr11
66685248
66729226
-
SYVN1
chr11
65121780
65134533
-
TAGLN
chr11
117199370
117207464
+
TNFRSF10B
chr8
23020133
23069031
-
TULP4
chr6
158232236
158511828
+
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001727
chr7
99621041
99621930
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC040162.3
chr16
67882461
67886367
+
AC234582.1
chr1
155195004
155205495
+
KCNQ1OT1
chr11
2608328
2699994
-
LINC01521
chr22
31346777
31348719
+
MALAT1
chr11
65497688
65506516
+
NEAT1
chr11
65422774
65445540
+
NORAD
chr20
36045618
36051018
-
SNHG22
chr18
49814023
49851059
+
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.