Entry Detail


General Information

Database ID:exR0257322
RNA Name:hsa-miR-326
RNA Type:miRNA
Chromosome:chr11
Starnd:-
Coordinate:
Start Site(bp):75335108End Site(bp):75335127
External Links:hsa-miR-326



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
DOLPP1
chr9
129081111
129090438
+
GOSR2
chr17
46923075
46975524
+
ITGA5
chr12
54395261
54419266
-
MAP4
chr3
47850690
48089272
-
NME4
chr16
396725
410367
+
PAQR4
chr16
2969270
2973484
+
RC3H1
chr1
173931084
174022357
-
REXO1
chr19
1815248
1848483
-
RTL10
chr22
19846138
19854896
-
STX3
chr11
59713456
59805882
+
TSPAN14
chr10
80454166
80533124
+
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000128
chr1
151139409
151139890
+
hsa_circ_0000384
chr12
27867712
27877119
+
hsa_circ_0001727
chr7
99621041
99621930
+
hsa_circ_0001865
chr9
86292641
86293514
-
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC016876.2
chr17
7581964
7584086
-
AC144548.1
chr12
110387463
110445548
-
AL355075.4
chr14
20343048
20343685
-
AL359924.1
chr1
237862175
237928321
+
AP000553.1
chr22
21652270
21670237
+
KCNQ1OT1
chr11
2608328
2699994
-
MIRLET7BHG
chr22
46053869
46113928
+
NEAT1
chr11
65422774
65445540
+
SNHG1
chr11
62851984
62855953
-
SNHG3
chr1
28505980
28510892
+
TUG1
chr22
30969245
30979395
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.