Entry Detail


General Information

Database ID:exR0257338
RNA Name:hsa-miR-339-5p
RNA Type:miRNA
Chromosome:chr7
Starnd:-
Coordinate:
Start Site(bp):1022990End Site(bp):1023012
External Links:hsa-miR-339-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ABL2
chr1
179099330
179229684
-
ARHGDIA
chr17
81867721
81871378
-
ATXN7
chr3
63864557
64003462
+
EDEM1
chr3
5187646
5219958
+
FEM1A
chr19
4791734
4801273
+
FOXP1
chr3
70952817
71583993
-
G3BP2
chr4
75642782
75724525
-
MMD
chr17
55392622
55421924
-
NCOA6
chr20
34689097
34825651
-
NUSAP1
chr15
41320794
41381050
+
PRDM15
chr21
41798225
41879482
-
PTPA
chr9
129110950
129148946
+
SLC44A1
chr9
105244622
105439171
+
STAT3
chr17
42313324
42388568
-
VCP
chr9
35056064
35072627
-
WDR4
chr21
42843094
42879568
-
ZNF689
chr16
30602558
30624012
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000120
chr1
117944807
118009049
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC004069.1
chr4
105137280
105140619
-
AC145207.5
chr17
81878425
81881106
-
GAS5
chr1
173858559
173868882
-
H19
chr11
1995176
2001470
-
KCNQ1OT1
chr11
2608328
2699994
-
LINC01578
chr15
92819540
92899701
+
NEAT1
chr11
65422774
65445540
+
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.