Entry Detail


General Information

Database ID:exR0257342
RNA Name:hsa-miR-33b-5p
RNA Type:miRNA
Chromosome:chr17
Starnd:-
Coordinate:
Start Site(bp):17813897End Site(bp):17813916
External Links:hsa-miR-33b-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ADCY9
chr16
3953387
4116442
-
AFF4
chr5
132875395
132963634
-
BTBD7
chr14
93237550
93333092
-
CERK
chr22
46684410
46738252
-
CROT
chr7
87345681
87399795
+
DDX5
chr17
64498254
64508199
-
E2F3
chr6
20401879
20493714
+
ECHDC1
chr6
127288712
127343609
-
EIF5A2
chr3
170888418
170908644
-
IGF1R
chr15
98648539
98964530
+
IRS2
chr13
109752695
109786583
-
MAPK1IP1L
chr14
55051647
55070194
+
MAPK6
chr15
51952106
52067375
+
MTHFD2
chr2
74198610
74217565
+
MYO19
chr17
36495633
36543435
-
NAA15
chr4
139301505
139420033
+
NFAT5
chr16
69565094
69704666
+
PIM1
chr6
37170152
37175428
+
RBM33
chr7
155644451
155781485
+
RCAN1
chr21
34513142
34615113
-
ROBO1
chr3
78597239
79767998
-
SLC39A8
chr4
102251041
102431258
-
SULF2
chr20
47656348
47786616
-
TNPO1
chr5
72816312
72916733
+
TPM1
chr15
63042632
63071915
+
UBN2
chr7
139230356
139308236
+
WDFY3
chr4
84669610
84966391
-
XRN1
chr3
142306607
142448062
-
ZFYVE16
chr5
80408013
80483379
+
ZNF148
chr3
125225561
125375354
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000540
chr14
55799534
55821940
+
hsa_circ_0001349
chr3
152132729
152150709
+
hsa_circ_0000642
chr15
80390757
80415142
+
hsa_circ_0000643
chr15
80412669
80415142
+
hsa_circ_0000284
chr11
33307958
33309057
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC023509.1
chr12
53441741
53467528
+
DANCR
chr4
52712404
52720351
+
FGD5-AS1
chr3
14920347
14948424
-
MCF2L-AS1
chr13
112967484
112968824
-
NEAT1
chr11
65422774
65445540
+
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.