Entry Detail


General Information

Database ID:exR0257348
RNA Name:hsa-miR-345-5p
RNA Type:miRNA
Chromosome:chr14
Starnd:+
Coordinate:
Start Site(bp):100307876End Site(bp):100307897
External Links:hsa-miR-345-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC011455.2
chr19
38915404
38949855
-
CNBP
chr3
129169484
129183922
-
FAM222B
chr17
28755978
28855232
-
GCNT4
chr5
75025346
75052558
-
GPD2
chr2
156435290
156613735
+
HEG1
chr3
124965710
125055997
-
IGF2BP1
chr17
48997385
49056145
+
LCLAT1
chr2
30447226
30644225
+
MAP3K14
chr17
45263119
45317029
-
MAPK1
chr22
21754500
21867680
-
RCOR1
chr14
102592649
102730561
+
RIPOR1
chr16
67518418
67546788
+
SARS2
chr19
38915266
38930896
-
SPTBN2
chr11
66685248
66729226
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001867
chr9
86294689
86297981
-
hsa_circ_0001866
chr9
86293355
86297981
-
hsa_circ_0001392
chr4
3088665
3109150
+
hsa_circ_0001387
chr4
1902352
1936989
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC079781.5
chr7
97851688
97972985
-
GAS5
chr1
173858559
173868882
-
KCNQ1OT1
chr11
2608328
2699994
-
LINC01772
chr1
16460948
16468481
+
NEAT1
chr11
65422774
65445540
+
SNHG15
chr7
44983023
44986961
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.