Entry Detail


General Information

Database ID:exR0257362
RNA Name:hsa-miR-3609
RNA Type:miRNA
Chromosome:chr7
Starnd:+
Coordinate:
Start Site(bp):98881700End Site(bp):98881723
External Links:hsa-miR-3609



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
IGF2
chr11
2129112
2141238
-
MAP3K7
chr6
90513573
90587072
-
MYH9
chr22
36281277
36388067
-
PIK3C2A
chr11
17077730
17207983
-
CCNY
chr10
35247025
35572669
+
CCT2
chr12
69585426
69601570
+
GPRIN3
chr4
89236383
89307800
-
ING5
chr2
241687085
241729478
+
NUP98
chr11
3671083
3797792
-
P2RX5-TAX1BP3
chr17
3663063
3696194
-
PBX2
chr6
32184733
32190202
-
RPLP0
chr12
120196699
120201235
-
TAX1BP3
chr17
3662895
3668679
-
WDR74
chr11
62832342
62841809
-
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AL356488.2
chr1
109100193
109100619
+
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.