Entry Detail


General Information

Database ID:exR0257381
RNA Name:hsa-miR-362-5p
RNA Type:miRNA
Chromosome:chrX
Starnd:+
Coordinate:
Start Site(bp):50008968End Site(bp):50008991
External Links:hsa-miR-362-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ATP2B4
chr1
203626561
203744081
+
BCL9L
chr11
118893875
118925608
-
BTBD1
chr15
83016423
83067252
-
CREG1
chr1
167529117
167553805
-
GLIS2
chr16
4314761
4339597
+
GRAMD4
chr22
46576012
46679790
+
NRIP1
chr21
14961235
15065936
-
STK4
chr20
44966479
45080021
+
TEX261
chr2
70985942
70994873
-
WDR4
chr21
42843094
42879568
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001247
chr22
46125304
46136418
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
KCNQ1OT1
chr11
2608328
2699994
-
LINC00324
chr17
8220642
8224043
-
LINC00641
chr14
21200079
21206900
-
SNHG16
chr17
76557764
76565348
+
TUG1
chr22
30969245
30979395
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.