exRNAdisease

Entry Detail

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General Information

Database ID: exR0257392 RNA Name: hsa-miR-3648 RNA Type: miRNA Chromosome: chr21 Starnd: + Coordinate: Start Site(bp): 8987026 End Site(bp): 8987046 External Links: hsa-miR-3648

Disease Information

Disease Name: Obesity Disease Category: Other Diseases MeSH ID: D009765 Type: Diseases Category/Nutritional and Metabolic Diseases Alias: Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics

Expression Detail

GEO ID: GSE125494 Description: Adipocyte-Derived Exosomal microRNAs in subject serum samples Experimental Design: Disease vs Control Case Disease Type: Obesity Case Disease SubType: NA Case Sample: Obesity Control Sample: Control Number of Case: 8 Number of Control: 3 Number of Samples: 11

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand MT-CO2 chrMT 7586 8269 + THAP7 chr22 20999104 21002196 - CERS4 chr19 8206736 8262421 + ARL8B chr3 5122245 5180912 + EEF1G chr11 62559596 62574086 - FNBP4 chr11 47716494 47767443 - PTGES3 chr12 56663341 56688408 - AP002990.1 chr11 62559603 62591531 - PTCH2 chr1 44819844 44843253 - MAPK9 chr5 180233143 180292099 - ACTG1 chr17 81509971 81523847 - ECPAS chr9 111360692 111484745 - RNF126 chr19 647526 663233 - SF3A2 chr19 2236824 2248655 + TPT1 chr13 45333471 45341370 - AKIRIN2 chr6 87674860 87702233 - LZTS3 chr20 3162617 3173592 - AL133352.1 chr10 100505628 100529881 - ATP6V0A2 chr12 123712353 123761755 + SET chr9 128683424 128696400 + SH3GLB2 chr9 129007036 129028303 - PAM16 chr16 4331549 4355607 - LRRC45 chr17 82023305 82031151 + SRRM2 chr16 2752626 2772538 + NDUFB8 chr10 100523740 100530000 - GAPDH chr12 6534512 6538374 + CORO7-PAM16 chr16 4340251 4420494 - SKI chr1 2228319 2310213 + SSR2 chr1 156009048 156020951 - ARRDC4 chr15 97960703 97973833 + miRNA targets: NA circRNA targets: NA lncRNA targets: NA Display:

Experiment Detail

GEO ID: GSE125494 Sample Source: Blood Source Fraction: Exosome Platform: GPL21572 Method: Microarray Num of detected RNA Type: 1 Num of detected RNAs of this Type: 2546 Sample treatment protocol: To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes. RNA Extract protocol: Total RNA was isolated following mirVana's total RNA Isolation kit. RNA library preparation protocol: biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample

Reference

PMID: 31331347 Title: Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs Author: Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ Journal: J Transl Med. 2019 Jul 22;17(1):232. Description: We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.